Urease from Canavalia ensiformis (Jack bean)
SIGMA/U1500 - Type III, powder, 15,000-50,000 units/g solid
Synonym: Jack bean urease; Urea amidohydrolase
CAS Number: 9002-13-5
EC Number: 232-656-0
MDL Number: MFCD00070858
Product Type: Chemical
application(s) | diagnostic assay manufacturing |
biological source | Canavalia ensiformis |
form | powder |
mol wt | ~544620 Da |
Quality Level | 300 ![]() |
specific activity | 15,000-50,000 units/g solid |
storage temp. | 2-8°C |
type | Type III |
Biochem/physiol Actions: | Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects. |
General description: | Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620 |
General description: | Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926. |
Packaging: | 20000, 100000, 500000, 1000000 units in poly bottle |
Unit Definition: | One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C) |
Symbol | ![]() ![]() |
Signal word | Danger |
Hazard statements | H315 - H319 - H334 - H335 |
Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
Hazard Codes | Xn |
Risk Statements | 36/37/38-42 |
Safety Statements | 22-24-26-36/37 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
activity | specific activity: 15,000-50,000 units/g solid |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 3.5.1.5 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352204 |