Urease from Canavalia ensiformis (Jack bean)
SIGMA/U1875 - Type III, glycerol solution, 500-800 units/mL
Synonym: Jack bean urease; Urea amidohydrolase
CAS Number: 9002-13-5
MDL Number: MFCD00070858
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: U1875-100ML.
| biological source | Canavalia ensiformis |
| concentration | 500-800 units/mL |
| form | glycerol solution |
| mol wt | ~544620 |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| type | Type III |
| Application: | Urease from Canavalia ensiformis (Jack bean) has been used: • to assay creatine and creatine phosphate using the sample extracted from the left ventricle (LV) and septal tissue • to determine the concentration of urea in a stabilized urine sample • as one of the standards to measure the urease activity of plant extracts |
| Biochem/physiol Actions: | In plants, ureases are essential for the bioavailability/metabolis |
| Biochem/physiol Actions: | Urease activity has been shown to be inhibited by benzpyrene following exposure to light. This inhibition was due to the effect of benzpyrene on urease′s sulfhydryl groups. |
| General description: | Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620 |
| General description: | Urease of jack bean (Canavalia ensiformis), a hexamer of a single chain of 840 amino acid residues, is found in plants, bacteria, fungi, algae, and soil. It is considered the first nickel-containing enzyme and the first enzyme to be crystallized. Ureases exist in several isoforms, some are jack bean urease (JBURE-I), canatoxin (CNTX), and JBURE-IIB. JBURE-I is more abundant than the other two isoforms. Ureases are found in mature seeds. |
| Packaging: | 100 mL in glass bottle |
| Unit Definition: | One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C) |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 23-26-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |