DMT-2′O-TBDMS-rU Phosphoramidite
SIGMA/U211000
Synonym: 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-[(1,1-dimethylethyl)dimethylsilyl]-uridine, 3′-[2-cyanoethyl N,N-
CAS Number: 118362-03-1
Empirical Formula (Hill Notation): C45H61N4O9PSi
Molecular Weight: 861.05
MDL Number: MFCD00080343
Linear Formula: C45H61N4O9PSi
Product Type: Chemical
| λ | conforms (UV/VIS Identity) |
| assay | ≥99% (31P-NMR) |
| ≥99.0% (reversed phase HPLC) | |
| biological source | non-animal source (no BSE/TSE risk) |
| color | white to off-white |
| form | powder |
| impurities | ≤0.3 wt. % water content (Karl Fischer) |
| ≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
| ≤0.5% single Impurity (reversed phase HPLC) | |
| ≤3 wt. % residual Solvent content | |
| InChI | 1S/C45H61N4O9PSi/c1-31(2) |
| InChI key | SKNLXHRBXYGJOC-ZMHKPELYSA |
| nucleoside profile | base: uridine base protecting group: none 2' protecting group: TBDMS 5' protecting group: DMT deprotection: fast/standard |
| product line | Proligo Reagents |
| Quality Level | 200  |
| SMILES string | COc1ccc(cc1)C(OC[C@H]2O[C |
| storage temp. | −20°C |
| suitability | conforms to structure for H-NMR |
| conforms to structure for LC-MS |
| Application: | RNA interference (RNAi) has become a popular tool for the sequence-specific inhibition of gene expression and can be used in target validation and other drug development techniques. The most convenient method to provide sequence-specific RNA oligonucleotides is a chemical synthesis on a solid support with RNA phosphoramidites and RNA CPG, analogous to DNA synthesis. |
| Features and Benefits: | • Industry-standard 2′O-TBDMS protective group • Consistent lot-to-lot purity and performance • Compatible with deprotection methods based on methylamine or AMA • Standard RNA phosphoramidites provide excellent coupling results when used with ETT or BTT as activator; best results are obtained with Activator 42 • Capping with standard acetic anhydride capping reagent rather than with Fast Deprotection Cap A • Manufactured under a certified ISO 9001 quality system |
| General description: | DMT-2′O-TBDMS-rU Phosphoramidite belongs to the group of 2′O-TBDMS RNA Phosphoramidites. |
| Other Notes: | The unique properties of RNA have stimulated the development of a variety of applications in diagnostics and therapeutics, as well as in basic molecular biology research where RNA can be used as: • Catalytic agents (ribozymes) • Affinity ligands (aptamers) • Agents to induce gene silencing (RNA interference) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |