DMT-2′O-TBDMS-rU Phosphoramidite
SIGMA/U211050 - configured for ÄKTA® and OligoPilot®
Synonym: 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-[(1,1-dimethylethyl)dimethylsilyl]-uridine, 3′-[2-cyanoethyl N,N-
CAS Number: 118362-03-1
Empirical Formula (Hill Notation): C45H61N4O9PSi
Molecular Weight: 861.05
MDL Number: MFCD00080343
Linear Formula: C45H61N4O9PSi
Product Type: Chemical
| λ | conforms (UV/VIS Identity) |
| assay | ≥99% (31P-NMR) |
| ≥99.0% (reversed phase HPLC) | |
| biological source | non-animal source (no BSE/TSE risk) |
| color | white to off-white |
| compatibility | configured for ÄKTA® and OligoPilot® |
| form | powder |
| impurities | ≤0.3% water content (Karl Fischer) |
| ≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
| ≤0.5% single Impurity (reversed phase HPLC) | |
| ≤3% residual Solvent content | |
| InChI | 1S/C45H61N4O9PSi/c1-31(2) |
| InChI key | SKNLXHRBXYGJOC-ZMHKPELYSA |
| nucleoside profile | base: uridine base protecting group: none 2' protecting group: TBDMS 5' protecting group: DMT deprotection: fast/standard |
| product line | Proligo Reagents |
| Quality Level | 200  |
| SMILES string | COc1ccc(cc1)C(OC[C@H]2O[C |
| storage temp. | −20°C |
| suitability | conforms to structure for H-NMR |
| conforms to structure for LC-MS | |
| technique(s) | oligo synthesis: suitable |
| General description: | RNA phosphoramidites, along with RNA CPG, and analogous to DNA synthesis support the synthesis of sequence-specific RNA oligonucleotides for RNA interference (RNAi) and can be used in target validation and other drug development techniques.Modern phosphoramidite-mediated synthesis has enabled routine high-yielding preparations of RNA oligonucleotides. High-quality RNA phosphoramidites are key to low failure rates, the high biological activity of synthesis products, and cost-effectiveness.Key Features: • Industry-standard 2′O-TBDMS protective group • Consistent lot-to-lot purity and performance • Compatible with deprotection methods based on methylamine or AMA • Standard RNA phosphoramidites provide excellent coupling results whenused with ETT or BTT as an activator; best results are obtained withActivator 42 • Capping with standard acetic anhydride capping reagent rather than withFast Deprotection Cap A • Manufactured under a certified ISO 9001 quality systemDMT-2′O-TBDMS-rU Phosphoramidite is configured for ABI™ Synthesizers. |
| Legal Information: | ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | OligoPilot is a registered trademark of Cytiva |
| Other Notes: | RNA monomers feature hydroxyl groups at the 2′-position. In order toprevent the formation of unnatural 2′-5′ phosphordiester bonds duringchain elongation, the 2′OH group is protected with a trialkyl-silyl group, tertbutyldimethylsilyl (TBDMS). The TBDMS group is stable under the acidicconditions used to remove the DMT group during the synthesis cycle, butcan be removed by a variety of methods after cleavage and deprotection ofthe RNA oligomer, e.g., with a solution of tetrabutylammonium fluoride(TBAF) in tetrahydrofurane (THF) or with triethylamine hydrofluoride. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |