DMT-2′O-Methyl-rU Phosphoramidite
SIGMA/U211140 - configured for MerMade
Synonym: 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-uridine, 3′-[2-cyanoethyl N,N-
CAS Number: 110764-79-9
Empirical Formula (Hill Notation): C40H49N4O9P
Molecular Weight: 760.81
MDL Number: MFCD00792626
Linear Formula: C40H49N4O9P
Product Type: Chemical
assay | ≥99% (31P-NMR) |
≥99.0% (reversed phase HPLC) | |
biological source | non-animal source (no BSE/TSE risk) |
color | white to off-white |
compatibility | configured for MerMade |
form | powder |
impurities | ≤0.3% mU2 (reversed phase HPLC, Hydrolysate) |
≤0.3% mU3 (reversed phase HPLC, DMT-rme) | |
≤0.3% water content (Karl Fischer) | |
≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
≤0.5% single Impurity (redox titration) | |
≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT) | |
≤3% residual Solvent content | |
InChI | 1S/C40H49N4O9P/c1-27(2)44 |
InChI key | UVUOJOLPNDCIHL-XKZJCBTISA |
product line | Proligo Reagents |
Quality Level | 200 ![]() |
SMILES string | CO[C@@H]1[C@H](OP(OCCC#N) |
storage temp. | 2-8°C |
suitability | conforms to structure for H-NMR |
conforms to structure for LC-MS |
Application: | 2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of: • Diagnostic probes • Aptamer and ribozyme development • Mixed 2′O-Methyl-RNA/DNA antisense molecules |
General description: | DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites. Its key features include:• High yield of crude oligonucleotides Compatible with DNA synthesis • Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides • Recommended deprotection conditions are 8 hours at 55 deg C using concentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 deg C • Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation • Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6minutes compared to 90 seconds for DNA monomers) • 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |