DMT-2′O-Methyl-rU Phosphoramidite
SIGMA/U211150 - configured for ÄKTA® and OligoPilot®
Synonym: 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-uridine, 3′-[2-cyanoethyl N,N-
CAS Number: 110764-79-9
Empirical Formula (Hill Notation): C40H49N4O9P
Molecular Weight: 760.81
MDL Number: MFCD00792626
Linear Formula: C40H49N4O9P
Product Type: Chemical
| λ | conforms (UV/VIS Identity) |
| assay | ≥99% (31P-NMR) |
| ≥99.0% (reversed phase HPLC) | |
| biological source | non-animal source (no BSE/TSE risk) |
| color | white to off-white |
| compatibility | configured for ÄKTA® and OligoPilot® |
| form | powder |
| impurities | ≤0.1% single unspecified Impurity (reversed phase HPLC) |
| ≤0.3% mU2 (reversed phase HPLC, Hydrolysate) | |
| ≤0.3% mU3 (reversed phase HPLC, DMT-rme) | |
| ≤0.3% water content (Karl Fischer) | |
| ≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
| ≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT) | |
| ≤3% residual Solvent content | |
| InChI | 1S/C40H49N4O9P/c1-27(2)44 |
| InChI key | UVUOJOLPNDCIHL-XKZJCBTISA |
| nucleoside profile | base: uridine base protecting group: none 2' protecting group: methyl 5' protecting group: DMT deprotection: fast/standard |
| product line | Proligo Reagents |
| Quality Level | 300  |
| SMILES string | CO[C@@H]1[C@H](OP(OCCC#N) |
| storage temp. | -10 to -25°C |
| suitability | conforms to structure for H-NMR |
| conforms to structure for LC-MS | |
| technique(s) | oligo synthesis: suitable |
| Application: | 2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance. |
| Features and Benefits: | Its key features include: • High yield of crude oligonucleotides • Compatible with DNA synthesis • Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides • Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA (concentrated ammonia/ 40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C • Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation • Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers) • 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry |
| General description: | DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | OligoPilot is a registered trademark of Cytiva |
| Other Notes: | The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of: • Diagnostic probes • Aptamer and ribozyme development • Mixed 2′O-Methyl-RNA/DNA antisense molecules |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
| Storage Temp. | -10 to -25°C |
| UNSPSC | 41116105 |