DMT-2′Fluoro-dU Phosphoramidite
SIGMA/U211200
Synonym: DMT-2′-Fluoro-dU amidite
CAS Number: 146954-75-8
Empirical Formula (Hill Notation): C39H46FN4O8P
Molecular Weight: 748.78
MDL Number: MFCD11113919
Linear Formula: C39H46FN4O8P
Product Type: Chemical
λ | conforms (UV/VIS Identity) |
assay | ≥99% (31P-NMR) |
≥99.0% (reversed phase HPLC) | |
biological source | non-animal source (no BSE/TSE risk) |
color | white to off-white |
form | powder |
impurities | ≤0.3% water content (Karl Fischer) |
≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
≤0.5% single Impurity (reversed phase HPLC) | |
≤3% residual Solvent content | |
InChI | 1S/C39H46FN4O8P/c1-26(2)4 |
InChI key | HQHQPAYRJJMYQX-DJTPZYMWSA |
nucleoside profile | base: deoxyuridine base protecting group: none 2' protecting group: fluoro 5' protecting group: DMT deprotection: fast/standard |
product line | Proligo Reagents |
Quality Level | 200 ![]() |
SMILES string | COc1ccc(cc1)C(OC[C@H]2O[C |
storage temp. | −20°C |
suitability | conforms to structure for H-NMR |
conforms to structure for LC-MS | |
technique(s) | oligo synthesis: suitable |
Application: | DMT-2′Fluoro-dU Phosphoramidite is suitable for use in the synthesis of 2′-fluoro modified oligonucleotide sequences by employing automated solid-phase chemistry. |
General description: | DMT-2′Fluoro-dU Phosphoramidite is a 2′Fluoro phosphoramidite that is used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance. Its key features are- • Can be employed together with DNA or RNA phosphoramidites • Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C • Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers) • Consistent lot-to-lot high purity and performance • Manufactured under a certified ISO 9001 quality system |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |