DMT-2′Fluoro-dU Phosphoramidite
SIGMA/U211250 - configured for (ÄKTA® and OligoPilot®)
Synonym: DMT-2′-Fluoro-dU amidite
CAS Number: 146954-75-8
Empirical Formula (Hill Notation): C39H46FN4O8P
Molecular Weight: 748.78
MDL Number: MFCD11113919
Linear Formula: C39H46FN4O8P
Product Type: Chemical
| λ | conforms (UV/VIS Identity) |
| assay | ≥99% (31P-NMR) |
| ≥99.0% (reversed phase HPLC) | |
| biological source | non-animal source (no BSE/TSE risk) |
| color | white to off-white |
| compatibility | configured for (ÄKTA® and OligoPilot®) |
| form | powder |
| impurities | ≤0.3% water content (Karl Fischer) |
| ≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
| ≤0.5% single Impurity (reversed phase HPLC) | |
| ≤3% residual Solvent content | |
| InChI | 1S/C39H46FN4O8P/c1-26(2)4 |
| InChI key | HQHQPAYRJJMYQX-DJTPZYMWSA |
| nucleoside profile | base: deoxyuridine base protecting group: none 2' protecting group: fluoro 5' protecting group: DMT deprotection: fast/standard |
| product line | Proligo Reagents |
| Quality Level | 300  |
| SMILES string | COc1ccc(cc1)C(OC[C@H]2O[C |
| storage temp. | −20°C |
| suitability | conforms to structure for H-NMR |
| conforms to structure for LC-MS | |
| technique(s) | oligo synthesis: suitable |
| Application: | DMT-2′Fluoro-dU Phosphoramidite is suitable for use in the synthesis of 2′-fluoro modified oligonucleotide sequences by employing automated solid-phase chemistry. |
| General description: | DMT-2′Fluoro-dU Phosphoramidite is a 2′Fluoro phosphoramidite used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.Its key features are-• Can be employed together with DNA or RNA phosphoramidites • Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C • Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers) • Consistent lot-to-lot high purity and performance • Manufactured under a certified ISO 9001 quality system |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | OligoPilot is a registered trademark of Cytiva |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |