Ubiquitin Carrier Protein H3/CDC34 human
SIGMA/U2383 - ≥95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain
MDL Number: MFCD03101856
Product Type: Chemical
| application(s) | cell analysis |
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | solution |
| mol wt | ~32 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli overproducing strain |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P49427  |
| P61088 |
| Biochem/physiol Actions: | Cdc34 is an essential gene for viability in yeast. Cdc34 mediates the transition from G1 to S-phase of the cell cycle by degrading the S-phase cyclin/CDK inhibitor, SIC1 in yeast and p27(KIP1) in human. Cdc34 is also known to interact with the Skp1/Cdc53/F-box (SCF) ubiquitin ligase subunits Cul1 to degrade the NF-κB inhibitor IκBα in a phosphorylation-dependent manner. |
| Biochem/physiol Actions: | Infected cell protein 0 of herpes simplex virus 1 was shown to bind UbcH3 and induce its degradation by promoting its polyubiquination. This resulted in increased expression of an UbcH3-target gene cyclin D1 and aided in further viral infection. |
| Other Notes: | Manufactured for Sigma by Boston Biochem. Inc. |
| Physical form: | Solution in 50 mM HEPES, 200 mM NaCl, 1 mM TCEP (pH 7.5), and 10% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |