Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat

SIGMA/U3254 - clone DECMA-1, ascites fluid, buffered aqueous solution

Synonym: Anti-E-Cadherin; Anti-LCAM

MDL Number: MFCD00164755
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-U3254-100UL	100 µL	$541.00 1/EA	Add To Favorites
45-U3254-.2ML	0.2 mL	$678.00 1/EA	Add To Favorites

Western Blotting JOURNAL CITATION: Claudin-3 overexpression increases the malignant potential of colorectal cancer cells: roles of ERK1/2 and PI3K-Akt as modulators of EGFR signaling. By: de Souza, W. F., Fortunato-Miranda, N., et al. in PLoS One, 2013. PubMed ID: 24069372 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0074994), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Immunohistochemistry-immunofluorescence JOURNAL CITATION: Alx4 relays sequential FGF signaling to induce lacrimal gland morphogenesis. By: Garg, A., Bansal, M., et al. in PLoS Genet, 2017. PubMed ID: 29028795 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1007047), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Immunofluorescence MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 (Cat. No. U3254) diluted to 1:3,200. The antibody was developed using Rabbit Anti-Rat IgG, FITC-conjugate.

Immunofluorescence MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3,200 Rat Monoclonal Anti-Uvomorulin/E-Cadherin, Clone: DECMA-1 (Cat. No. U3254). The antibody was developed using Goat Anti-Rat IgG, FITC conjugate.

Immunoblotting MDCK cell extract was separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 (Cat. No. U3254). The antibody was developed using Rabbit Anti-Rat IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:250

Properties
Descriptions
Safety Info.
Basic Data

antibody form	ascites fluid
antibody product type	primary antibodies
biological source	rat
clone	DECMA-1, monoclonal
conjugate	unconjugated
contains	15 mM sodium azide
form	buffered aqueous solution
isotype	IgG1
Quality Level	200
shipped in	dry ice
species reactivity	bovine, human, canine, mouse
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	immunohistochemistry (frozen sections): suitable
	immunoprecipitation (IP): suitable
	indirect immunofluorescence: 1:1,600 using cultured MDCK cells
	microarray: suitable
	western blot: suitable
UniProt accession no.	P12830

Application:	Monoclonal Anti-Uvomorulin/E-Cadherin has been used in immunofluorescence, immunoblotting, immunoprecipitation, immunohistochemistry, macromolecule permeability assay and agglomeration of two embryoid body assay.
Biochem/physiol Actions:	Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.
Biochem/physiol Actions:	The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in immunoblotting or immunohistochemical assays.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	Monoclonal Anti-Uvomorulin/E-Cadherin (rat IgG1 isotype) is derived from the DECMA-1 hybridoma, produced by the fusion of rat myeloma cells and splenocytes from an immunized Lou rat. Uvomorulin protein was initially identified in embryonal carcinoma and is identical to E-Cadherin, liver-cell adhesion molecules (L-CAM), Cell CAM 80/120, and Activity-regulated cytoskeleton-associated protein 1 (ARC-1), each of which have been characterized in different systems. Uvomorulin/E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca²⁺.
Immunogen:	mouse embryonal carcinoma cell line PCC4 Aza R1.

Hazard Codes	Xn
Risk Statements	21/22
Safety Statements	36/37-60
RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203

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