Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat
SIGMA/U3254 - clone DECMA-1, ascites fluid, buffered aqueous solution
Synonym: Anti-E-Cadherin; Anti-LCAM
MDL Number: MFCD00164755
Product Type: Chemical
Western Blotting JOURNAL CITATION: Claudin-3 overexpression increases the malignant potential of colorectal cancer cells: roles of ERK1/2 and PI3K-Akt as modulators of EGFR signaling. By: de Souza, W. F., Fortunato-Miranda, N., et al. in PLoS One, 2013. PubMed ID: 24069372 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0074994), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Alx4 relays sequential FGF signaling to induce lacrimal gland morphogenesis. By: Garg, A., Bansal, M., et al. in PLoS Genet, 2017. PubMed ID: 29028795 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1007047), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Alx4 relays sequential FGF signaling to induce lacrimal gland morphogenesis. By: Garg, A., Bansal, M., et al. in PLoS Genet, 2017. PubMed ID: 29028795 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1007047), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 (Cat. No. U3254) diluted to 1:3,200. The antibody was developed using Rabbit Anti-Rat IgG, FITC-conjugate.
Immunofluorescence MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3,200 Rat Monoclonal Anti-Uvomorulin/E-Cadherin, Clone: DECMA-1 (Cat. No. U3254). The antibody was developed using Goat Anti-Rat IgG, FITC conjugate.
Immunoblotting MDCK cell extract was separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 (Cat. No. U3254). The antibody was developed using Rabbit Anti-Rat IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:250
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | DECMA-1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, human, canine, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (frozen sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect immunofluorescence: 1:1,600 using cultured MDCK cells | |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P12830  |
| Application: | Monoclonal Anti-Uvomorulin/E-Cadheri |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Uvomorulin/E-Cadheri |
| Immunogen: | mouse embryonal carcinoma cell line PCC4 Aza R1. |
| Specificity: | Monoclonal Anti-Uvomorulin/E-Cadheri |
| Specificity: | The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in immunoblotting or immunohistochemical assays. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |