Urease Buffer Reagent
SIGMA/U3383
Synonym: Urea colorimetric determination assay reagent
Product Type: Chemical
| description | When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative. |
| form | powder |
| mol wt | 545 kDa |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Urease Buffer Reagent has been used: • to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method • in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells • to study the metabolic capacity of rat hepatocytes |
| Application: | Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea. |
| General description: | Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia. |
| General description: | Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants. Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections. two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 |
| Precautionary statements | P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 21/22-42 |
| Safety Statements | 23-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |