Urease from Canavalia ensiformis (Jack bean)
SIGMA/U4002 - Type IX, powder, 50,000-100,000 units/g solid
Synonym: Jack bean urease; Urea amidohydrolase
CAS Number: 9002-13-5
EC Number: 232-656-0
MDL Number: MFCD00070858
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: U4002-1MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: U4002-100KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: U4002-20KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: U4002-500KU
| form | powder |
| mol wt | ~544620 Da |
| Quality Level | 200  |
| specific activity | 50,000-100,000 units/g solid |
| storage temp. | 2-8°C |
| type | Type IX |
| Application: | Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis. |
| Biochem/physiol Actions: | In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline. |
| Biochem/physiol Actions: | Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide. |
| General description: | Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620 |
| General description: | Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms. |
| Packaging: | 20000, 100000, 500000, 1000000 units in poly bottle |
| Unit Definition: | One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C) |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| activity | specific activity: 50,000-100,000 units/g solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |