Anti-Urocortin antibody produced in rabbit
SIGMA/U4757 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD01632996
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Delineation of motoneuron subgroups supplying individual eye muscles in the human oculomotor nucleus. By: Che Ngwa, E., Zeeh, C., et al. in Front Neuroanat, 2014. PubMed ID: 24574976 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/article/10.3389/fnana.2014.00002/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Delineation of motoneuron subgroups supplying individual eye muscles in the human oculomotor nucleus. By: Che Ngwa, E., Zeeh, C., et al. in Front Neuroanat, 2014. PubMed ID: 24574976 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/article/10.3389/fnana.2014.00002/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 1:100 Anti-Urocortin antibody produced in Rabbit (Cat. No. U4757) followed by 10 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using microwave-treated sections of human placental tissue |
| quantitative precipitin assay: 1:10,000 using 125I-Tyr0-Urocortin (rat) | |
| UniProt accession no. | P55089  |
| Application: | Anti-Urocortin antibody produced in rabbit has been used in: • radioimmunoassay (RIA) • immunohistochemistry • immunocytochemistry |
| Application: | Anti-urocortin antibody produced in rabbit has been used to evaluate the entry of urocortin peptide into the brain. |
| Biochem/physiol Actions: | Urocortin (UCN) plays a vital role in inhibiting transforming growth factor β1 (TGFβ1) oncogenic signaling pathway. The encoded protein is implicated in systemic and peripheral stress-response systems. Mutations in UCN gene might increase the risk of susceptibility to obesity. |
| Biochem/physiol Actions: | Urocortin has biological actions like corticotropin-releasing factor (CRF) such as autonomic and behavioral responses in mammalian species. It is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary in vitro and in vivo. In the central nervous system (CNS), urocortin acts to produce potent anxiogenic-like and appetite suppressing effects and peripherally has hypotensive effects. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Urocortin (UCN) is a neuropeptide, widely distributed in the central nervous system (CNS). Urocortin is derived from a larger precursor protein (pro-urocortin, also named corticotensin). |
| General description: | Urocortin (UCN) is encoded by the gene mapped to human chromosome 2p21–23. The encoded protein belongs to the corticotropin-releasing hormone (CRH) family. UCN is a 40 amino acid long peptide. It is widely expressed in the heart, lung, skin and immune cells. |
| Immunogen: | synthetic peptide corresponding to the C-terminal of human urocortin (amino acids 25-40 with N-terminally added lysine) conjugated to KLH. The immunogen sequence (human) corresponds to pro-urocortin (amino acids 105-120) and is identical in rat urocortin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Specificity: | The antibody is evaluated for activity and specificity by radioimmunoassay (RIA) and immunohistochemistry. The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, PACAP38, human ACTH, human ACTH (18-39), and sauvagine. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |