Anti-U2AF65 antibody, Mouse monoclonal
SIGMA/U4758 - clone MC3, purified from hybridoma cell culture
Synonym: Anti-U2AF65
MDL Number: MFCD03456068
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.2 μg/mL Monoclonal Anti-U2AF65 Clone: MC3 (Cat. No. U4758). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. HEK-293T 3. HepG2 4. A431 5. AT3B1 6. LA-4
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MC3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~65 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, Xenopus, mouse |
| storage temp. | −20°C |
| technique(s) | electron microscopy: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.1-0.2 μg/mL using total cell extract from HeLa cells | |
| UniProt accession no. | P26368  |
| Application: | Monoclonal Anti-U2AF65 antibody produced in mouse has been used in: • RNA immunoprecipitation • immunoblotting • chromatin immunoprecipitation (ChIP) • enzyme-linked immunosorbent assay (ELISA) • immunohistochemistry • immunocytochemistry • immunoelectron microscopy |
| Biochem/physiol Actions: | U2 snRNP auxiliary factor 65 kDa (U2AF65)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF65 primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF65) and (U2AF35) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-U2AF65 (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF65. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF65) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF65kDa subunit is a non-snRNP protein component of spliceosome complexes. |
| Immunogen: | recombinant human U2AF65. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |