Urea
SIGMA/U4883 - 8 M (after reconstitution with 16 mL high purity water)
Synonym: Urea solution; Carbamide; Carbonyldiamide
CAS Number: 57-13-6
Empirical Formula (Hill Notation): CH4N2O
Molecular Weight: 60.06
EC Number: 200-315-5
MDL Number: MFCD00008022
Linear Formula: NH2CONH2
Product Type: Chemical
concentration | 8 M (after reconstitution with 16 mL high purity water) |
form | powder |
InChI | 1S/CH4N2O/c2-1(3)4/h(H4,2 |
InChI key | XSQUKJJJFZCRTK-UHFFFAOYSA |
Quality Level | 100 |
SMILES string | NC(N)=O |
solubility | H2O: soluble (1 vial plus 16 mL) |
Application: | Useful for solubilizing and denaturing proteins in preparation for 2D-PAGE, enzymatic digests, IMAC Chromatography, and related procedures. Urea has been used for protein sample preparation for reverse-phase nanospray liquid chromatography-tandem mass spectrometry. |
General description: | Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. It can also disturb hydrogen bonding present in DNA secondary structure. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12352200 |