Urea
SIGMA/U5378 - powder, BioReagent, for molecular biology, suitable for cell culture
Synonym: Carbamide; Carbonyldiamide
CAS Number: 57-13-6
Empirical Formula (Hill Notation): CH4N2O
Molecular Weight: 60.06
EC Number: 200-315-5
MDL Number: MFCD00008022
Linear Formula: NH2CONH2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: U5378-100G
This image depicts SKU: U5378-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: U5378-500G
This image depicts SKU: U5378-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: U5378-1KG
This image depicts SKU: U5378-1KG
| absorption | ≤0.06 at 260 at 6 M |
| ≤0.06 at 280 at 6 M | |
| anion traces | chloride (Cl-): ≤5 ppm |
| assay | ≥98% |
| cation traces | Cu: ≤1 ppm |
| Fe: ≤5 ppm | |
| Pb: ≤2 ppm | |
| density | 1.335 g/mL at 25 °C (lit.) |
| foreign activity | DNase, NICKase, RNase and protease, none detected |
| form | powder |
| grade | for molecular biology |
| InChI | 1S/CH4N2O/c2-1(3)4/h(H4,2 |
| InChI key | XSQUKJJJFZCRTK-UHFFFAOYSA |
| mp | 132-135 °C (lit.) |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | NC(N)=O |
| solubility | H2O: soluble 480 mg/mL, clear, colorless (8M) |
| technique(s) | cell culture | mammalian: suitable |
| Application: | • Urea has been used in protein resuspension buffer and to denature proteins. • It has been used for the isolation of proteins via urea-containing polyacrylamide gels. • It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH. |
| Application: | Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100, 500 g in poly bottle |
| Packaging: | 5 kg in poly drum |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% |
| mp | 132-135 °C (lit.) |
| Density | 1.335 g/mL at 25 °C (lit.) |
| UNSPSC | 12352200 |