Anti-Ubiquitin antibody produced in rabbit
SIGMA/U5379 - whole antiserum, lyophilized powder
MDL Number: MFCD00162897
Product Type: Chemical
antibody form | whole antiserum |
antibody product type | primary antibodies |
biological source | rabbit |
clone | polyclonal |
conjugate | unconjugated |
form | lyophilized powder |
mol wt | antigen 8.6 kDa |
Quality Level | 200 ![]() |
species reactivity | prokaryotes, eukaryotes |
storage temp. | 2-8°C |
technique(s) | immunohistochemistry (frozen sections): suitable |
western blot: 1:100 using ubiquitin purified from bovine red blood cells | |
UniProt accession no. | P0CG47 ![]() ![]() |
Application: | Anti-Ubiquitin antibody produced in rabbit is used in: • immunostaining in neuropathology • immunohistochemistry • western blotting |
Biochem/physiol Actions: | Ubiquitin is involved in the selective degradation of many short-lived proteins by 26S proteasome. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays crucial roles in the regulation of various processes, such as cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, stress response, protein trafficking and endocytosis. Ubiquitin protein also aids in immune response, metabolism, development, normal protein turnover, protein quality control by degrading misfolded and damaged proteins and apoptosis. Variation in the ubiquitin-mediated processes results in carcinogenesis. |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | Ubiquitin (UBB) gene is mapped to human chromosome 17p11.2. |
General description: | Ubiquitin is a highly conserved, 8.6kDa, eukaryotic cellular protein containing 76 amino acid residues. Ubiquitin is absent in prokaryotes. |
Immunogen: | bovine red blood cell ubiquitin. |
Physical form: | Lyophilized from 0.01 M phosphate buffer, containing 1% lactose. |
Reconstitution: | Reconstitute the contents of the vial with 10 mL of Tris buffered saline. Since the antisera is preservative free, it is recommended that it be reconstituted in the presence of a preservative or aliquoted and frozen. Avoid repeated freeze/thaw cycles. |
Specificity: | By immunoblotting, the antibody recognizes 1-3 protein bands using bovine red blood cell ubiquitin. |
Target description: | Ubiquitin, present in both prokaryotes and eukaryotes, is a highly conserved 8.6 kDa protein. It is involved in the selective degradation of many short-lived proteins by the proteasome, an ATP-dependent protease. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays important roles in the control of numerous processes, including cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, and endocytosis. It has also been implicated in immune response, metabolism, development, protein quality control, and apoptosis. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352203 |