Ubiquitin human
SIGMA/U5382 - ≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), essentially salt-free, lyophilized powder
CAS Number: 60267-61-0
MDL Number: MFCD02100852
Product Type: Chemical
The ubiquitin-proteasome system. Ubiquitin is first activated by the ubiquitin-activating enzyme, E1, to a high energy thiol ester intermediate. It is then transferred, still as a high energy intermediate, to a member of the ubiquitin-carrier proteins family of enzymes, E2 (also known as a ubiquitin-conjugating enzyme - UBC). From E2 it can be transferred directly to the substrate, S, that is bound specifically to a member of the ubiquitin ligase family of proteins, E3. This occurs when the E3 belongs to the RING finger family of ligases (A). In the case of an HECT (Homologous tothe E6-AP C-Terminal domain) domain-containing E3 ligase (B), the activated ubiquitin moiety is transferred first to the E3, to generate yet another high-energy thiol ester intermediate, before it is transferred to the E3-bound target substrate. Additional ubiquitin moieties are added successively to
the previously conjugated one in a similar mechanism to generate a poly-ubiquitin chain. The poly-ubiquitinated substrate is bound specifically to the
26S proteasome complex. The substrate is degraded to short peptides, and free and reusable ubiquitin is released via the activity of de-ubiquitinating
enzymes (DUBS)
the previously conjugated one in a similar mechanism to generate a poly-ubiquitin chain. The poly-ubiquitinated substrate is bound specifically to the
26S proteasome complex. The substrate is degraded to short peptides, and free and reusable ubiquitin is released via the activity of de-ubiquitinating
enzymes (DUBS)
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | essentially salt-free, lyophilized powder |
| mol wt | 10 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli (N-terminal FLAG® tagged) |
| solubility | 0.05 M Tris pH 7.5: ≥10 mg/mL |
| storage temp. | −20°C |
| technique(s) | mass spectrometry (MS): suitable |
| UniProt accession no. | P0CG47  |
| P0CG48 |
| Application: | Ubiquitin human can be used as a standard for mass spectrometry. |
| Application: | Ubiquitin human has been used in mono-ubiquitination of yeast proliferating cell nuclear antigen (PCNA) and in in vitro ubiquitination assay of defective in mitotic arrest 1 (Dma1) |
| Application: | Ubiquitin, N-terminal FLAG-tagged can replace the ubiquitin in formation of poly-ubiquitin—protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blot by anti-FLAG antibodies. |
| Biochem/physiol Actions: | Ubiquitin interacts with the lysine residue of proteins through its ε-amino group of the C-terminal glycine residue. Proteins interacting with ubiquitin either undergo mono-ubiquitination or multi-mono-ubiquitination via a three-step process. Ubiquitination regulates intracellular trafficking and protein degradation and an imbalance in the pathway is implicated in disorders. |
| Biochem/physiol Actions: | Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway. |
| General description: | Ubiquitin is a highly conserved globular protein and has lysine in the surface. The C-terminal end comprises the Leu-Arg-Gly-Gly structural motif. |
| Legal Information: | FLAG is a registered trademark of Sigma-Aldrich Co. LLC |
| Packaging: | 1 mg in poly bottle |
| Packaging: | Package size based on protein content |
| Preparation Note: | Ubiquitin human can be dissolved in 0.05 M Tris-HCl at a concentration of 10.00 - 11.00 mg/ml to yield a clear to slightly hazy, colorless solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |