Ubiquitin−Agarose
SIGMA/U5632 - saline suspension
CAS Number: 60267-61-0
MDL Number: MFCD02100851
Product Type: Chemical
The ubiquitin-proteasome system. Ubiquitin is first activated by the ubiquitin-activating enzyme, E1, to a high energy thiol ester intermediate. It is then transferred, still as a high energy intermediate, to a member of the ubiquitin-carrier proteins family of enzymes, E2 (also known as a ubiquitin-conjugating enzyme - UBC). From E2 it can be transferred directly to the substrate, S, that is bound specifically to a member of the ubiquitin ligase family of proteins, E3. This occurs when the E3 belongs to the RING finger family of ligases (A). In the case of an HECT (Homologous tothe E6-AP C-Terminal domain) domain-containing E3 ligase (B), the activated ubiquitin moiety is transferred first to the E3, to generate yet another high-energy thiol ester intermediate, before it is transferred to the E3-bound target substrate. Additional ubiquitin moieties are added successively to
the previously conjugated one in a similar mechanism to generate a poly-ubiquitin chain. The poly-ubiquitinated substrate is bound specifically to the
26S proteasome complex. The substrate is degraded to short peptides, and free and reusable ubiquitin is released via the activity of de-ubiquitinating
enzymes (DUBS)
the previously conjugated one in a similar mechanism to generate a poly-ubiquitin chain. The poly-ubiquitinated substrate is bound specifically to the
26S proteasome complex. The substrate is degraded to short peptides, and free and reusable ubiquitin is released via the activity of de-ubiquitinating
enzymes (DUBS)
| extent of labeling | 7-15 mg per mL |
| form | saline suspension |
| matrix | Fast flow highly cross-linked 4% beaded agarose |
| matrix spacer | 6 carbon |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| Application: | Ubiquitin-agarose is used in affinity chromatography, protein chromatography, intracellular protein degradation, calpain and lysosomal proteases, proteomics, specialty resins and ubiquitination analysis. Ubiquitin-agarose has been used in a study to produce evidence for a particulate location of ubiquitin conjugates and ubiquitin-conjugating enzymes in the rabbit brain. Ubiquitin-agarose has also been used to study fertilization of the ascidian, Halocynthia roretzi. |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Suspension in 1 M NaCl containing 15 Mm Sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |