Ubiquitin from bovine erythrocytes
SIGMA/U6253 - BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder
Synonym: ATP-dependent proteolytic factor; Ub
CAS Number: 79586-22-4
MDL Number: MFCD00082121
Product Type: Chemical
SDS-PAGE (4-20%) of ubiquitin (Cat. No. U6253) under reducing conditions and stained with Coomassie blue.
| assay | ≥98% (SDS-PAGE) |
| biological source | bovine erythrocytes |
| extent of labeling | (Report results) |
| form | essentially salt-free, lyophilized powder |
| mol wt | 8560 Da by calculation (monoisotopic mass) |
| 8565 Da by calculation (average mass) | |
| product line | BioUltra |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | western blot: suitable |
| UniProt accession no. | A0A3Q1M4K3  |
| Application: | Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay. The product can also be used as a marker in western blotting. |
| Biochem/physiol Actions: | Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway. |
| General description: | A 76-amino acid protein (8.6 kDa) that is bound covalently by a conjugating enzyme to proteins that are targeted for degradation by 26S proteasome. Occurs in virtually all eukaryotes, including plants (thus, its name). The sequence is highly conserved; identical in animal sources from insects to humans. |
| Packaging: | 25 mg in poly bottle |
| Packaging: | 5 mg in glass bottle |
| Preparation Note: | Ubiquitin from bovine erythrocytes can dissolved in water at 1 mg/ml to yield a clear, colorless solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |