Anti-UCP-1 antibody produced in rabbit
SIGMA/U6382 - affinity isolated antibody, buffered aqueous solution
Synonym: Ucp1 Antibody; Ucp1 Antibody - Anti-UCP-1 antibody produced in rabbit; Anti-Uncoupling Protein 1
MDL Number: MFCD01092514
Product Type: Chemical
Western Blotting JOURNAL CITATION: Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression. By: Nam, M., Akie, T. E., et al. in Sci Rep, 2017. PubMed ID: 28515438 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-01879-x), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression. By: Nam, M., Akie, T. E., et al. in Sci Rep, 2017. PubMed ID: 28515438 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-01879-x), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression. By: Nam, M., Akie, T. E., et al. in Sci Rep, 2017. PubMed ID: 28515438 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-01879-x), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of Mouse brown adipose tissue stained with 1:500 Anti-UCP-1 antibody produced in Rabbit (Cat. No. U6382) followed by Anti-Rabbit IgG-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting Extract of rat brown fat was separated on SDS-PAGE, blotted with Anti-UCP1 (Cat. No. U6382) and developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase (Cat. No. A9919) and a colorimetric substrate. Lanes 1. Antibody 1 μg/mL 2. Antibody 1 μg/mL + UCP1 immunizing peptide (human, 145-159)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~32 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of mouse brown adipose tissue. |
| western blot: 1:1,000 using mitochondrial extract of rat brown adipose tissue | |
| UniProt accession no. | P25874  |
| Application: | Anti-UCP-1 antibody produced in rabbit has been used in western blotting and immunohistochemistry. |
| Biochem/physiol Actions: | Uncoupling proteins (UCPs) are transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They can return protons pumped by the respiratory chain to the mitochondrial matrix. UCP-1 is exclusively expressed in brown adipose tissue (BAT) in rodents and in neonates where it is regulated by norepinephrine and thyroid hormones. UCP-1 has been shown to depend on CoQ (ubiquinone) as an obligatory co-factor. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Uncoupling protein (UCP1) is a 32 kDa, mitochondrial inner membrane protein. It is active as a proton channel forming dimer. It can bind purine nucleotides and is capable of being stimulated by fatty acids. |
| Immunogen: | synthetic peptide corresponding to human UCP1 sequence (amino acids 145-159) with N-terminal cysteine added, conjugated to KLH. The corresponding sequence is identical in rat and mouse UCP1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Specificity: | Anti-UCP-1 specifically recognizes human, mouse and rat UCP-1. Staining of the UCP-1 band is specifically inhibited with the immunizing peptide. The epitopes recognized are compatible with routine formalin-fixation and paraffin-embedding. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |