Ubiquitin-Specific Protease 14 human
SIGMA/U6385 - recombinant, expressed in Sf9 cells, ≥75% (SDS-PAGE)
Product Type: Chemical
| assay | ≥75% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | 57 kDa |
| NCBI accession no. | NM_005151   |
| Quality Level | 200 |
| recombinant | expressed in Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human USP14 (Ubiquitin-Specific Protease 14) or TGT (tRNA-guanine transglycosylase), amino acids 2-494 (end) with an N-terminal FLAG-tag, expressed in a baculovirus-infected Sf9 cell expression system. Ubiquitin-Specific Protease 14 is a deubiquitinating protein responsible for the removal of ubquitin tags attached to proteins by α•NH-peptide bonds and the ε-NH-isopeptide bond. |
| General description: | USP14 has been shown to be phosphorylated by AKT which is required for protein stability and nuclear localization. |
| Physical form: | Solution in 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 80 ng/μl Flag peptide, 3 mM DTT, and 20% Glycerol at pH 8.0. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥75% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |