Urea
SIGMA/U6504 - suitable for electrophoresis
Synonym: Carbamide; Carbonyldiamide
CAS Number: 57-13-6
Empirical Formula (Hill Notation): CH4N2O
Molecular Weight: 60.06
EC Number: 200-315-5
MDL Number: MFCD00008022
Linear Formula: NH2CONH2
Product Type: Chemical
absorption | ≤0.06 at 260 at 5 M |
≤0.06 at 280 at 5 M | |
assay | ≥98% |
cation traces | Fe: ≤5 ppm |
Pb: ≤5 ppm | |
density | 1.335 g/mL at 25 °C (lit.) |
form | powder or crystals |
impurities | ≤0.005% Ammonia |
InChI | 1S/CH4N2O/c2-1(3)4/h(H4,2 |
InChI key | XSQUKJJJFZCRTK-UHFFFAOYSA |
mp | 132-135 °C (lit.) |
Quality Level | 200 |
SMILES string | NC(N)=O |
solubility | H2O: 100 mg/mL |
technique(s) | electrophoresis: suitable |
Application: | A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis. |
Application: | Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. |
Packaging: | 1 kg in poly bottle |
Packaging: | 500 g in poly bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% |
mp | 132-135 °C (lit.) |
Density | 1.335 g/mL at 25 °C (lit.) |
UNSPSC | 12352111 |