Anti-UVRAG antibody produced in rabbit
SIGMA/U7508 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-UV radiation resistance-
Product Type: Chemical
Immunoblotting Whole extract of Mouse Brain was separated on SDS-PAGE and probed with Rabbit Anti-UVRAG (Cat. No. U7508). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + UVRAG immunizing peptide (human, 665-678)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~90 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2-4 μg/mL using whole extracts of mouse brain and human Hela cells |
| UniProt accession no. | Q9P2Y5  |
| Application: | Anti-UVRAG antibody has been used in: • immunoblotting • western blotting • immunofluorescence |
| Application: | Anti-UVRAG antibody produced in rabbit has been used: • in western blotting • in immunofluorescence |
| Biochem/physiol Actions: | Ultraviolet irradiation resistance-associated gene (UVRAG) is implicated in the regulation of intracellular membrane trafficking, including autophagy and chromosomal stability. The encoded protein regulates apoptosis by suppressing the BCL2-associated X protein (Bax) activity. Mutation in the gene has been observed in various types of human cancers, including microsatellite unstable colon carcinomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ultraviolet irradiation resistance-associated gene (UVRAG) is an important autophagic tumor suppressor, encoded by the gene mapped to human chromosome 11q13.5. The encoded protein is characterized with four functional domains, such as proline-rich domain, a lipid-binding C2 domain, a beclin1-binding coiled-coil domain (CCD) and a C-terminal domain involved in centrosome integrity and DNA damage repair. |
| Immunogen: | synthetic peptide corresponding to amino acids 665-678 of human UVRAG, conjugated to KLH. The corresponding sequence is identical in mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Target description: | UVRAG activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. This gene complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a prot |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |