Monoclonal Anti-USP25 antibody produced in mouse
SIGMA/U7883 - ~1.0 mg/mL, clone U25.7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-USP21; Anti-Ubiquitin specific peptidase 25
Product Type: Chemical
Immunoblotting Whole lysate of HEK-293 cells was separated on SDS-PAGE and probed with Monoclonal Anti-USP25, Clone: U25.7 (Cat. No. U7883). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL 2. 2 μg/mL+ non-immunizing peptide 3. 2 μg/mL+ USP25 immunizing peptide
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | U25.7, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~126 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 1-2 μg/mL using total extract of HEK-293T cells expressing USP25. | |
| UniProt accession no. | Q9UHP3  |
| Application: | Monoclonal Anti-USP25 antibody has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunocytochemistry |
| Biochem/physiol Actions: | USP25 (ubiquitin-specific protease 25) is a target for sumoylation. It takes place within the two ubiquitin interaction motifs (UIM) of USP25 that are required for efficient hydrolysis of Ub (ubiquitin), and thus impairs USP25 activity as a result of reduced affinity to Ub chains. Overexpression of USP25 has been observed in Down syndrome fetal brain. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-USP25 (mouse IgG2a isotype) is derived from the hybridoma U25.7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human USP25. USP25 (ubiquitin-specific protease 25) is a member of the ubiquitin processing proteases (UBP) family. It is located on the gene-poor region of 21q11.2 human chromosome. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |