Anti-ULK2 antibody produced in rabbit
SIGMA/U8008 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Ulk2 Antibody; Ulk2 Antibody - Anti-ULK2 antibody produced in rabbit; Anti-Unc-51 like kinase 2; Anti-Unc51.2
Product Type: Chemical
Immunoblotting Whole extract of human HEK-293T cells overexpressing mouse ULK2 was separated on SDS-PAGE and probed with Rabbit Anti-ULK2 (Cat. No. U8008). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 5 μg/mL antibody 3. 5 μg/mL antibody + ULK2 immunizing peptide (mouse, 370-382)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~130 kDa |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells expressing mouse ULK2 |
| UniProt accession no. | Q9QY01   |
| Application: | Anti-ULK2 antibody produced in rabbit has been used in western blot analysis and immunohistochemistry. |
| Biochem/physiol Actions: | Uncoordinated family member (unc)-51 like autophagy activating kinase 2 (ULK2) plays a crucial role in autophagy. It is also implicated in the regulation of elemental biological processes conserved among wide variety of species. Downregulated expression of the gene increases astrocyte transformation and tumor growth. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Uncoordinated family member (unc)-51 like autophagy activating kinase 2 (ULK2) is encoded by the gene mapped to mouse chromosome 11. Mouse ULK2 (mULK2) belongs to the UNC-51-like protein kinase family. mULK2 protein is characterized with an amino-terminal kinase domain, a central proline/serine rich (PS) domain and a carboxy-terminal (C) domain. ULK2 is ubiquitously expressed and is mainly localized to autophagosomal membranes in mammalian cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |