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Uridine-5′-diphosphoglucose pyrophosphorylase from baker’s yeast

SIGMA/U8501 - Type X, lyophilized powder, ≥40 units/mg protein

Synonym: UTP:α-D-glucose-1-phosphate uridylyltransferase

CAS Number: 9026-22-6
EC Number: 232-811-2
MDL Number: MFCD00132561
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-U8501-25UN 25 units
 $168.00
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45-U8501-100UN 100 units
 $487.00
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45-U8501-500UN 500 units
 $1590.00
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45-U8501-1KU 1000 units
 $2330.00
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biological source bakers yeast
composition Protein, 30-60% modified Warburg-Christian
foreign activity inorganic pyrophosphatase ≤0.5%
  UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
form lyophilized powder
Quality Level 200 
specific activity ≥40 units/mg protein
storage temp. −20°C
type Type X
Application: Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:• in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
• to quantify Suc synthase (SUS) enzyme activity in rice
• to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes
Biochem/physiol Actions: Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.
General description: Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.
Other Notes: One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.
Physical form: Lyophilized, sulfate-free powder containing citrate buffer salt
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: ≥40 units/mg protein
Storage Temp. −20°C
Enzyme Commission (EC) Number 2.7.7.9   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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