Anti-Vascular Endothelial Growth Factor B 167/186 antibody produced in goat
SIGMA/V1010 - affinity isolated antibody, lyophilized powder
Synonym: Anti-VEGF-B 167/186
MDL Number: MFCD03097360
Product Type: Chemical
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 100  |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: 0.5-1.0 μg/mL |
| western blot: 0.1-0.2 μg/mL | |
| UniProt accession no. | P49765  |
| Application: | Anti-Vascular Endothelial Growth Factor B 167/186 antibody may be used for immunoblotting at a working antibody concentration of 0.1-0.2 μg/ml. For ELISA, a concentration of 0.5-1.0 μg/ml is recommended. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The vascular endothelial growth factor family consists of five members, VEGF-A to VEGF-E. VEGF-B has a structure that is similar to VEGF-A and the placenta growth factor. It is highly expressed in most tissues and organs and most abundantly in heart. VEGF-B binds and signals via the VEGFR-1 receptor and neuropilin-1 coreceptor. Even though it belongs to the family of growth factors that induce angiogenesis and are necessary for formation of vasculature, VEGF-B differs in acting as a survival factor and less of angiogenic factor. The survival effect is pleotropic and also affects neurons, vascular cells and cardiac myocytes. There are two isoforms of VEGF-B that result from alternate splicing, VEGF-B 167 and VEGF-B 186. VEGF-B 167 binds heparin and is associated with extracellular matrix whereas VEGF-B 186 is a secreted form. Anti-Human Vascular Endothelial Growth Factor B recognizes human VEGF-B 167 and human VEGF-B 186. The antibody shows approximately 30% cross reactivity with recombinant mouse VEGF-B 165 and VEGF-B 186. It exhibits less than 5% cross reactivity with recombinant human VEGF-D and no cross reactivity with recombinant human VEGF and recombinant mouse VEGF. |
| Immunogen: | recombinant human vascular endothelial growth factor B 186 expressed in E. coli. Purified by passing goat sera over a human VEGF-B 186 affinity column and then passing the bound fraction over a human VEGF-B 167 affinity column. |
| Physical form: | Lyophilized from a 2 μm filtered solution in phosphate buffered saline. |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |