Anti-VDAC/Porin antibody produced in rabbit
SIGMA/V2139 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-
MDL Number: MFCD00282412
Product Type: Chemical
Immunoblotting
Whole cell extract of rat liver mitochondria was separated on SDS-PAGE, blotted with Rabbit Anti-VDAC1/Porin (Cat. No. V2139) and developed using Goat Anti-Rabbit IgG Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
(Lane A) Antibody dilution 1:2,000; (Lane B) Antibody dilution 1:3,000; (Lane C) Antibody dilution 1:4,000; (Lane D) Antibody dilution 1:2,000 + 20 μg/mL VDAC1/Porin peptide.
Whole cell extract of rat liver mitochondria was separated on SDS-PAGE, blotted with Rabbit Anti-VDAC1/Porin (Cat. No. V2139) and developed using Goat Anti-Rabbit IgG Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
(Lane A) Antibody dilution 1:2,000; (Lane B) Antibody dilution 1:3,000; (Lane C) Antibody dilution 1:4,000; (Lane D) Antibody dilution 1:2,000 + 20 μg/mL VDAC1/Porin peptide.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 31 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | microarray: suitable |
| western blot: 1:2,000 using rat liver mitochondria extract or rat liver extract | |
| UniProt accession no. | P21796  |
| Application: | Anti-VDAC/porin antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Voltage dependent anion channel 1 (VDAC1) plays an essential role in several cellular processes. It helps in transport of ions and metabolites between the mitochondria and cytosol of the cell. Oligomerization of this protein facilitates discharge of cytochrome c to the cytoplasm, leading to apoptosis. VDAC1 expression is associated with the development of human papillomavirus (HPV)-related cervical cancer. Thus, VDAC1 knock down can be considered as a potent therapeutic strategy for treating HPV-induced cervical disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Voltage dependent anion channel 1 (VDAC1) is a highly regulated β-barrel membrane protein, encoded by the gene mapped to human chromosome 5q31.1. The encoded protein is a member of VDAC protein family and is mainly localized in the outer mitochondrial membrane. |
| Immunogen: | synthetic peptide located at an internal putative cytoplasmic loop of human VDAC1/Porin (amino acids 152-169) conjugated to KLH. The sequence is identical in bovine and rabbit VDAC1, highly conserved in mouse and rat VDAC1 (single amino acid substitution), and has considerable homology (60-70%) with the human, rat, and mouse VDAC isoforms VDAC2 and VDAC3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |