Monoclonal Anti-Vimentin antibody produced in mouse
SIGMA/V2258 - clone LN-6, ascites fluid
Synonym: Anti-F5H288
MDL Number: MFCD00162904
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Py2T murine breast cancer cells, a versatile model of TGFβ:-induced EMT in vitro and in vivo. By: Waldmeier, L., Meyer-Schaller, N., et al. in PLoS One, 2012. PubMed ID: 23144919 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0048651), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Py2T murine breast cancer cells, a versatile model of TGFβ:-induced EMT in vitro and in vivo. By: Waldmeier, L., Meyer-Schaller, N., et al. in PLoS One, 2012. PubMed ID: 23144919 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0048651), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Py2T murine breast cancer cells, a versatile model of TGFβ:-induced EMT in vitro and in vivo. By: Waldmeier, L., Meyer-Schaller, N., et al. in PLoS One, 2012. PubMed ID: 23144919 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0048651), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Imna(-/-) mouse embryonic fibroblasts stained with Monoclonal Anti-Vimentin, clone LN-6 (Cat. No. V2258) at a 1:40 dilution (red), Anti-Actin (blue) and DAPI (yellow). From Shyam Khatau, Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Immunohistochemistry Formalin fixed, paraffin-embedded Human Appendix tissue sections were stained with 1:200 Monoclonal Anti-Vimentin antibody produced in mouse, Clone: LN-6 (Cat. No. V2258), followed by 20 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Vimentin, Clone: LN-6 (Cat. No. V2258). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. MG-63; 2. HeLa; 3. HepG2; 4. NIH-3T3; 5. PC-12; 6. COS-7; 7. CHO; 8. MDBK; 9. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LN-6, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | pig, mouse, sheep, human, bovine, rabbit, feline, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections |
| immunoprecipitation (IP): suitable | |
| indirect immunofluorescence: suitable using cultured cells | |
| western blot: suitable | |
| UniProt accession no. | P08670  |
| Application: | Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) . |
| Application: | Monoclonal Anti-Vimentin antibody produced in mouse has been used in western blotting and immunolabeling. |
| Biochem/physiol Actions: | Vimentin plays an important role in modulating cell growth. It plays a pivotal role in epithelial-mesenchymal transition. Vimentin acts as a potential target for anti-metastasis therapy for nasopharyngeal carcinoma (NPC). It regulates cellular architecture and tissue integrity. It is also involved in tumorigenesis and the metastatic spread of cancer. Vimentin provides stress resistance. It might be involved in neuritogenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vimentin belongs to the class of type III intermediate filament protein family. The gene is located on human chromosome 10p13. It is a cytoskeletal protein. Vimentin is mainly expressed in normal mesenchymal cells. |
| General description: | Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit. |
| Immunogen: | human thymic nuclear extract |
| Specificity: | Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |