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Monoclonal Anti-V5-Peroxidase antibody produced in mouse

SIGMA/V2260 - clone V5-10, purified immunoglobulin, lyophilized powder

Synonym: Monoclonal Anti-V5

MDL Number: MFCD03457579
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-V2260-1VL 1 vial
 $1020.00
1/EA		 Add To Favorites
Western Blotting Whole extract of 293T cells expressing V5 were separated on SDS-PAGE. The antibody was probed and developed with Monoclonal Anti-V5-Peroxidase antibody produced in Mouse, Clone: V5-10 (Cat. No. V2260). Lanes 1. 1:4,000 antibody 2. 1:8,000 antibody 3. Negative Control

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone V5-10, monoclonal
concentration 5-11 mg/mL
conjugate peroxidase conjugate
form lyophilized powder
packaging vial of 0.5 mL
Quality Level 200 
storage temp. 2-8°C
technique(s) western blot: 1:4,000-1:8,000
Application: Suitable for immunoblotting.
Biochem/physiol Actions: Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
General description: The V5 tag is a 14-amino acid long peptide sequence (GKPIPNPLLGLDST) that is derived from an epitope on the P and V proteins of simian virus 5 (SV5), which belongs to the paramyxovirus family. Monoclonal Anti-V5 Peroxidase conjugate reacts with V5-tagged recombinant fusion proteins expressed in transfected mmmalian cells or produced by in vitro translation.
Physical form: Lyophilized from a 0.01 M phosphate buffered saline solution, pH 7.4, containing 1% BSA and 0.05% MIT
Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Preparation Note: Reconstitute with 0.5 mL of distilled water.
Storage Temp. 2-8°C
UNSPSC 12352203

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