Monoclonal Anti-V5-Peroxidase antibody produced in mouse
SIGMA/V2260 - clone V5-10, purified immunoglobulin, lyophilized powder
Synonym: Monoclonal Anti-V5
MDL Number: MFCD03457579
Product Type: Chemical
Western Blotting Whole extract of 293T cells expressing V5 were separated on SDS-PAGE. The antibody was probed and developed with Monoclonal Anti-V5-Peroxidase antibody produced in Mouse, Clone: V5-10 (Cat. No. V2260). Lanes 1. 1:4,000 antibody 2. 1:8,000 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | V5-10, monoclonal |
| concentration | 5-11 mg/mL |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| packaging | vial of 0.5 mL |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | western blot: 1:4,000-1:8,000 |
| Application: | Suitable for immunoblotting. |
| General description: | The V5 tag is a 14-amino acid long peptide sequence (GKPIPNPLLGLDST) that is derived from an epitope on the P and V proteins of simian virus 5 (SV5), which belongs to the paramyxovirus family. Monoclonal Anti-V5 Peroxidase conjugate reacts with V5-tagged recombinant fusion proteins expressed in transfected mmmalian cells or produced by in vitro translation. |
| Physical form: | Lyophilized from a 0.01 M phosphate buffered saline solution, pH 7.4, containing 1% BSA and 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Preparation Note: | Reconstitute with 0.5 mL of distilled water. |
| Specificity: | Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |