Anti-Vanilloid Receptor-1 antibody produced in rabbit
SIGMA/V2764 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-TRPV1; Anti-VR-1
MDL Number: MFCD02686673
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Vanilloid Receptor-1 antibody produced in Rabbit (Cat. No. V2764) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Vanilloid Receptor-1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with 1:20,000 Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Western Blotting Whole extract of 293T cells expressing Rat VR-1 were separated on SDS-PAGE, probed and developed with Anti-Vanilloid Receptor-1 antibody produced in Rabbit (Cat. No. V2764) followed by 1:20,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control 4. Untransfected 293T cells, 1:1,000 antibody
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 90 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| technique(s) | microarray: suitable |
| western blot: 1:1,000 using whole cell extract of 293T cells expressing rat VR-1 | |
| UniProt accession no. | O35433  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vanilloid Receptor-1 (VR-1) is a cation channel protein that stimulates a burning pain sensation upon binding to capsaicin, a component of ′hot′ chilli peppers. VR-1 is also activated by noxious heat stimuli. Moreover, VR-1 can combine both, heat and chemical stimuli to cause a sensation of pain in vivo. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of rat vanilloid receptor-1 (amino acids 817-838) conjugated to KLH. The sequence is highly conserved in guinea pig (~85% identity) and human (~70% identity) and is not found in rat VRL-1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti-Vanilloid Receptor-1 antibody is specific for vanilloid receptor-1 (90 kDa) in rats. The vanilloid receptor-1 immunizing peptide (rat, amino acids 817-838) specifically inhibits the staining of the vanilloid receptor-1 band in immunoblotting. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |