Anti-V5-Cy3™ antibody, Mouse monoclonal
SIGMA/V4014 - purified immunoglobulin, ~1 mg/mL, clone V5-10
Synonym: Monoclonal Anti-V5
MDL Number: MFCD03457579
Product Type: Chemical
Immunofluorescence
Cytoplasmic localization of V5-HIS-LacZ fusion protein in transfected HEK-293T cells, stained with 2 μg/mL Monoclonal Anti-V5-Cy3 conjugate (Clone V5-10) (Cat. No. V4014).
Cytoplasmic localization of V5-HIS-LacZ fusion protein in transfected HEK-293T cells, stained with 2 μg/mL Monoclonal Anti-V5-Cy3 conjugate (Clone V5-10) (Cat. No. V4014).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | V5-10, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | CY3 conjugate |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: 2.0-4.0 μg/mL |
| Application: | Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence. |
| General description: | Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry. |
| Legal Information: | Cy3 is a trademark of Cytiva |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Specificity: | Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |