Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-1 antibody produced in mouse
SIGMA/V4262 - clone FLT-11, ascites fluid
Synonym: Anti-Flt1 Receptor; Anti-VEGF R-1
MDL Number: MFCD01634354
Product Type: Chemical
Immunoblotting Supernatant cultured insect cells with ERK2 fusion protein, C-terminal HA were separated on SDS-PAGE and probed with Monoclonal AB To VEGF Receptor-1 (FLT1 Receptor) Clone: FLT-11 (Cat. No. V4262). The antibody was developed with Alk Phos APA Mouse IgG ads HIgG (Cat. No. A1293) and NBT/BCIP substrate. Antibody dilution 1/1,000.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FLT-11, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 160-200 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1:500 using VEGF Receptor-1 (Flt-1)/Fc Chimera human recombinant | |
| UniProt accession no. | P17948  |
| Application: | Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-1 antibody can be used in several immunochemical assays like immunohistochemistry, immunocytochemistry and immunoprecipitation for the localization of VEGF-R1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vascular endothelial growth factor (VEGF) is also called vasculotropin (VAS) and vascular permeability factor (VPF). It is a homodimeric heparin-binding glycoprotein. |
| General description: | Vascular Endothelial Growth Factor Receptor-2 (VEGF R2) is a 160-200kD protein that belongs to protein kinase superfamily and is expressed mainly on the surface of various endothelial cells. It plays a pivotal role in the regulation of angiogenesis, cell migration, cell survival and cancer cell invasion. ASV derived from VEGF receptor type 1 serve as a potential therapeutics for rheumatoid arthritis. Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-1antibody can be used in western blotting (diluted 1:500) using VEGF Receptor-1 (Flt-1)/Fc Chimera human recombinant. It can also be used in indirect ELISA. Mouse anti-Vascular Endothelial Growth Factor Receptor-1antibody reacts specifically with an epitope within amino acids 1-251 of the extracellular domain of human VEGF Receptor-1 but not with VEGF Receptor-2 (KDR). The product has shown cross reactivity with the mouse Flt1 receptor. |
| Immunogen: | recombinant human VEGF-1 receptor. |
| Specificity: | Mouse monoclonal clone FLT-11 anti-VEGF R-1 antibody reacts specifically with the extracellular domain of human VEGF Receptor-1. The epitope recognized by the antibody resides within amino acid residues 1-251 of the VEGF Receptor-1 molecule. The product does not recognize VEGF Receptor-2 (KDR). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |