Monoclonal Anti-Vinculin antibody produced in mouse
SIGMA/V4505 - clone VIN-11-5, ascites fluid
Synonym: Vinculin Antibody Sigma; Anti-VCL
MDL Number: MFCD00162905
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Cellular mechano-environment regulates the mammary circadian clock. By: Yang, N., Williams, J., et al. in Nat Commun, 2017. PubMed ID: 28134247 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms14287), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Cellular mechano-environment regulates the mammary circadian clock. By: Yang, N., Williams, J., et al. in Nat Commun, 2017. PubMed ID: 28134247 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms14287), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Double labeling of an isolated smooth muscle cell (chicken gizzard) with Monoclonal Anti-Vinculin, clone no. VIN-11-5, (Cat. No. V4505) (green) and Anti-α-Actinin (red).
Immunofluorescence
Mouse adult fibroblast stained with Monoclonal Anti-Vinculin, clone VIN-11-5 (Cat. No. V4505) at a 1:40 dilution (aqua). Actin (purple) and nuclear DNA (blue) were stained using phalloidin-Alexa Fluor 568 and DAPI, respectively.
From Christopher M. Hale, Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Mouse adult fibroblast stained with Monoclonal Anti-Vinculin, clone VIN-11-5 (Cat. No. V4505) at a 1:40 dilution (aqua). Actin (purple) and nuclear DNA (blue) were stained using phalloidin-Alexa Fluor 568 and DAPI, respectively.
From Christopher M. Hale, Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Immunoblotting Chicken gizzard cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Vinculin (MO) CL: VIN11-5 (Cat. No. V4505). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/200.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | VIN-11-5, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen 116 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, human, chicken, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:50 using cultured chicken fibroblasts |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P18206  |
| Application: | Monoclonal Anti-Vinculin antibody produced in mouse has been used in: • western blotting. • immunofluorescence. • immunocytochemistry. |
| Biochem/physiol Actions: | Vinculin (VCL) is a cytoskeletal protein, which plays a vital role in regulation of cell-to-cell and cell to matrix junctions. VCL facilitates normal embryonic development and cardiac function. Aberration or mutation of the gene leads to cardiomyopathy. VCL also plays a crucial role in cell migration and morphogenesis. VCL might facilitate the binding of integrins and cadherins to the actin cytoskeleton. Vinculin plays an important role in integrin αIIbβ3 signaling and function, which is required for platelet aggregation. |
| Biochem/physiol Actions: | Vinculin is suggested to be a possible link between ends of the bundles of actin filaments and the plasma membrane. It has also been proposed that vinculin may be involved in the transmembrane induction of actin bundle formation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | The vinculin (VCL) gene containing 22 exons is mapped to human chromosome 10q22.1-q23. The gene codes for a 117kDa cytoskeletal protein present in adherens type cell junctions. VCL is a ubiquitously expressed cell-adhesion protein. The protein is characterized with a globular head and a tail domain, which are interconnected by a short proline-rich sequence, an intramolecular interaction between the head and tail conceal many ligand-binding sites in the protein. |
| Immunogen: | smooth muscle vinculin from chicken gizzard. |
| Other Notes: | This product can be found as purified product that was produced using cell culture hybridoma product. SAB4200729 Anti-Vinculin antibody, Mouse monoclonal clone VIN-11-5, purified from hybridoma cell culture |
| Specificity: | The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |