Anti-Vimentin antibody produced in goat
SIGMA/V4630 - whole antiserum
Synonym: Anti-F5H288
MDL Number: MFCD00145922
Product Type: Chemical
Immunofluorescence Formalin fixed, paraffin-embedded Human skin tissue sections were stained with 1:20 Anti-Vimentin antibody produced in Goat (Cat. No. V4630) followed by 1:20 Anti-Goat IgG (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F7367).
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| mol wt | antigen 58 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: 1:20 using formalin-fixed, paraffin-embedded sections of human tissue | |
| western blot: 1:50 using HS-68 human foreskin cell extract | |
| UniProt accession no. | P08670  |
| Application: | Anti-Vimentin antibody produced in goat has been used in following studies: • immunohistochemistry • western blotting • immunocytochemistry • flow cytometry |
| Application: | Anti-Vimentin antibody produced in goat has been used in Immunohistochemistry and Western blot analysis. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Chromatin immunoprecipitation (1 paper) Immunocytochemistry (1 paper) Immunofluorescence (1 paper) Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | Vimentin is known to promote epithelial to mesenchymal transition and contributes to metastasis in cancer such as ovarian cancer. Vimentin preserves cellular structure and tissue integrity. Vimentin is also known to bring about TNF-α (tumor necrosis factor-α) induced cell apoptosis. |
| Biochem/physiol Actions: | Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of the gene has been observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for treating these cancers. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1) induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments is implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. The protein interacts with LARP6 (la ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore mutations in the gene causes dominant and pulverulent cataract. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The VIM (vimentin) gene codes for an intermediate filament protein, that is expressed throughout mesenchymal cells. VIM gene is mapped to human chromosome 10p13. |
| General description: | Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain. |
| Immunogen: | vimentin from cultured human foreskin fibroblasts. |
| Specificity: | The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |