Anti-VSV-G antibody produced in rabbit
SIGMA/V4888 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Vesicular Stomatitis Virus glycoprotein
MDL Number: MFCD00164761
Product Type: Chemical
Immunofluorescence 293T Human embryonic kidney cells expressing VSV-G tagged fusion proteins were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2 μg/mL Anti-VSV-G antibody produced in Rabbit (Cat. No. V4888) followed by 1:40 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Western Blotting Whole extract of 293T cells expressing VSV-G were separated on SDS-PAGE and probed with Anti-VSV-G antibody produced in Rabbit (Cat. No. V4888). The antibody was developed using 1:15,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.1 μg/mL antibody 2. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mammals |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates) |
| indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells | |
| western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates |
| Application: | Anti-VSV-G antibody produced in rabbit has been used in western blotting and immunoprecipitation. |
| Application: | Antibody is suitable for immunoblotting, immunoprecipitation, and indirect immunofluorescence. immunocytochemistry. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Chromatin immunoprecipitation (1 paper) Western Blotting (1 paper) Western blotting following immunoprecipitation (1 paper) |
| Biochem/physiol Actions: | Vesicular stomatitis virus G (VSV G) is capable of stimulating cell-cell fusion upon low pH stimulation. The transmembrane region of VSV G protein is highly essential for the virus transition from the hemi-fusion to full fusion state. |
| General description: | The Anti-VSV-G rabbit antibody reacts with amino- and carboxyl-terminal VSV-G tagged fusion proteins. |
| General description: | Vesicular stomatitis viruses (VSV) belongs to the Vesiculovirus genus of the Rhabdoviridae family. Vesicular stomatitis virus G (VSV G) protein is considered to be the early recognized type III viral fusion protein. |
| Immunogen: | synthetic peptide corresponding to amino acids 497-511 (N-Tyr-Thr-Asp-Ile-Glu-Me |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |