Anti-VSV-G antibody produced in rabbit
SIGMA/V4888 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Vesicular Stomatitis Virus glycoprotein
MDL Number: MFCD00164761
Product Type: Chemical
antibody form | affinity isolated antibody |
antibody product type | primary antibodies |
biological source | rabbit |
clone | polyclonal |
concentration | ~1.0 mg/mL |
conjugate | unconjugated |
form | buffered aqueous solution |
Quality Level | 200 |
shipped in | dry ice |
species reactivity | mammals |
storage temp. | −20°C |
technique(s) | immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates) |
indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells | |
western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates |
Application: | Anti-VSV-G antibody produced in rabbit has been used in western blotting and immunoprecipitation. |
Application: | Antibody is suitable for immunoblotting, immunoprecipitation, and indirect immunofluorescence. immunocytochemistry. |
Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Chromatin immunoprecipitation (1 paper) Western Blotting (1 paper) Western blotting following immunoprecipitation (1 paper) |
Biochem/physiol Actions: | Vesicular stomatitis virus G (VSV G) is capable of stimulating cell-cell fusion upon low pH stimulation. The transmembrane region of VSV G protein is highly essential for the virus transition from the hemi-fusion to full fusion state. |
General description: | The Anti-VSV-G rabbit antibody reacts with amino- and carboxyl-terminal VSV-G tagged fusion proteins. |
General description: | Vesicular stomatitis viruses (VSV) belongs to the Vesiculovirus genus of the Rhabdoviridae family. Vesicular stomatitis virus G (VSV G) protein is considered to be the early recognized type III viral fusion protein. |
Immunogen: | synthetic peptide corresponding to amino acids 497-511 (N-Tyr-Thr-Asp-Ile-Glu-Me |
Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |