Monoclonal Anti-Vimentin antibody produced in mouse
SIGMA/V5255 - clone VIM-13.2, ascites fluid
Synonym: Anti-F5H288
MDL Number: MFCD00145922
Product Type: Chemical
Western Blotting JOURNAL CITATION: miR-200 enhances mouse breast cancer cell colonization to form distant metastases. By: Dykxhoorn, D. M., Wu, Y., et al. in PLoS One, 2009. PubMed ID: 19787069 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0007181), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence
JOURNAL CITATION: Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons. By: Oh, S. I., Park, H. S., et al. in ScientificWorldJournal, 2014. PubMed ID: 24991651
Image collected and cropped by CiteAb from the following publication, (http://www.hindawi.com/journals/tswj/2014/957548/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
JOURNAL CITATION: Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons. By: Oh, S. I., Park, H. S., et al. in ScientificWorldJournal, 2014. PubMed ID: 24991651
Image collected and cropped by CiteAb from the following publication, (http://www.hindawi.com/journals/tswj/2014/957548/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Flow cytometry/Cell sorting JOURNAL CITATION: Surface vimentin is critical for the cell entry of SARS-CoV. By: Yu, Y. T., Chien, S. C., et al. in J Biomed Sci, 2016. PubMed ID: 26801988 Image collected and cropped by CiteAb from the following publication, (http://www.jbiomedsci.com/content/23/1/14), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
A mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431) grown on coverslips, fixed and stained with Monoclonal Anti-Pan Cytokeratin, clone no. PCK-26 (Cat. No. C1801) (green); Monoclonal Anti-Vimentin, clone no. VIM-13.2 (Cat. No. V5255) (red); and Hoechst No. 33258 (Cat. No. B2883) (blue) nuclear stain.
From G. MacKenzie, Immunogen Inc., Cambridge, MA.
A mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431) grown on coverslips, fixed and stained with Monoclonal Anti-Pan Cytokeratin, clone no. PCK-26 (Cat. No. C1801) (green); Monoclonal Anti-Vimentin, clone no. VIM-13.2 (Cat. No. V5255) (red); and Hoechst No. 33258 (Cat. No. B2883) (blue) nuclear stain.
From G. MacKenzie, Immunogen Inc., Cambridge, MA.
Western Blotting Whole extract of Human forskin fibroblasts HS-68 cells were separated on SDS-PAGE and probed with Monoclonal Anti-Vimentin antibody produced in Mouse, Clone: VIM-13.2 (Cat. No. V5255). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | VIM-13.2, monoclonal |
| conjugate | unconjugated |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, rat, human, rabbit, frog, mouse |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 1:200 using cultured chicken fibroblasts |
| western blot: 1:500 using human fibroblasts cell extract | |
| UniProt accession no. | P08670  |
| Application: | Monoclonal anti-vimentin antibody can be used in the following: • Immunocytochemical localization of vimentin in normal and pathological tissue of mesenchymal origin. • It can also be used in immunoblotting • Immunofluorescence • Further, anti-vimentin antibody can be used to distinguish tumors and metastatic lesions derived from sarcomas, lymphomas, and melanomas. |
| Biochem/physiol Actions: | Vimentin has a crucial role in maintaining cell shape and motility. It also provides mechanical stress tolerance for mesenchymal cells. Vimentin induces fibroblast motility and promotes wound healing process. Vimentin filaments form a part of cytoskeleton filament system and therefore, support and secure the cell organelles and also maintain cellular integrity. Vimentin is associated with the process of initiation, progression, metastasis and epithelial-mesenchymal transition of cancer cells, thereby contributing to tumorigenesis. Upregulation of this gene is observed in various cancers including prostate cancer, gastrointestinal tumors, lung cancer, breast cancer, CNS tumors and malignant melanoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vimentin is a 53 kD type III intermediate filaments expressed in breast myoepithelial cells, osteocytes, langerhans cells of the skin, schwann cells and astrocytes. Monoclonal anti-vimentin antibody can be used in immunofluorescent labeling of frozen tissue sections to localize vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes. It can also be used in western blotting. Mouse anti-vimentin antibody reacts specifically with vimentin. This product has also shown best reactivity with cultured chicken fibroblasts and can also react with human and mouse cells or tissues.The protein monomers consist of α-helical rod, flanked by head and tail domains. The VIM gene is mapped to human chromosome 10p13. |
| Immunogen: | human foreskin fibroblasts |
| Other Notes: | This product can be found as purified product that was produced using cell culture hybridoma product. SAB4200716 Anti-Vimentin antibody, Mouse monoclonal clone VIM-13.2, purified from hybridoma cell culture |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |