Monoclonal Anti-VSV Glycoprotein antibody produced in mouse
SIGMA/V5507 - clone P5D4, ascites fluid
Synonym: Monoclonal Anti-VSV Glycoprotein
Product Type: Chemical
Western Blotting Whole extract of 293-T Vinclin tagged at C-terminal with VSV-G cells were separated on SDS-PAGE and probed with Monoclonal Anti-VSV Glycoprotein antibody produced in Mouse, Clone: P5D4 (Cat. No. V5507). The antibody was developed using 1:1,500 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1:100,000 antibody 2. 1:150,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | P5D4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 1:100,000 using whole cell extracts expressing VSV-G tagged fusion protein |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Flow cytometry/Cell sorting (1 paper)  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH. |
| Specificity: | The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein. |
| Storage and Stability: | For continuous use, store at 2 °C to 8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |