Anti-Vimentin antibody, Mouse monoclonal
SIGMA/V6389 - clone V9, purified from hybridoma cell culture
Synonym: Anti-Vimentin antibody, Mouse monoclonal
Product Type: Chemical
Western Blotting JOURNAL CITATION: Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models. By: Sobral, L. M., Sousa, L. O., et al. in Mol Cancer, 2014. PubMed ID: 24555657 Image collected and cropped by CiteAb from the following publication, (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-32), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models. By: Sobral, L. M., Sousa, L. O., et al. in Mol Cancer, 2014. PubMed ID: 24555657 Image collected and cropped by CiteAb from the following publication, (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-32), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat brain sections with 0.05 μg/mL Monoclonal Anti-Vimentin antibody produced in mouse, Clone: V9 (Cat. No. V6389). The antibody was developed using goat-Anti-Mouse IgG, DyLight 594 conjugate. (The picture is courtesy of Fabrice GANKAM MD, Ph D., Néphrologie Dialyse et Transplantation, Hopital Erasme - ULB)
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.2 μg/mL Monoclonal Anti-Vimentin Clone: V9 (Cat. No. V6389). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HS-68 2. HeLa 3. U-2-OS 4. HEK-293T 5. COS7 6. RAT2
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | V9, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~58 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human |
| storage temp. | −20°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 25-50 μg/mL using human tissue | |
| microarray: suitable | |
| western blot: 0.2-0.5 μg/mL using human fibroblasts HS-68 | |
| UniProt accession no. | P08670  |
| Application: | Monoclonal Anti-Vimentin antibody produced in mouse has been used in immunohistochemical analysis and western blot assay. |
| Application: | Monoclonal Anti-Vimentin antibody produced in mouse has been used in: • immunofluorescence • immunohistochemistry • immunocytochemistry • immunoblotting • flow cytometry. |
| Biochem/physiol Actions: | Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the V9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with vimentin purified from pig eye lens. |
| General description: | Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α--helical amino- terminal “head” and carboxy-terminal “tail” domain. |
| Immunogen: | pig eye lens vimentin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |