Monoclonal Anti-Vimentin antibody produced in mouse
SIGMA/V6630 - clone V9, ascites fluid
Synonym: Monoclonal Anti-Vimentin
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Molecular mechanism for USP7-mediated DNMT1 stabilization by acetylation. By: Cheng, J., Yang, H., et al. in Nat Commun, 2015. PubMed ID: 25960197 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/ncomms8023), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Porcine Pluripotent Stem Cells Derived from IVF Embryos Contribute to Chimeric Development In Vivo. By: Xue, B., Li, Y., et al. in PLoS One, 2016. PubMed ID: 26991423 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0151737), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Molecular mechanism for USP7-mediated DNMT1 stabilization by acetylation. By: Cheng, J., Yang, H., et al. in Nat Commun, 2015. PubMed ID: 25960197 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/ncomms8023), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
Endothelial cells labeled with Monoclonal Anti-Vimentin (Cat. No. V6630) (green) and Anti-JNK2 (red).
From Lara Milone, Biochimica e Biofisica, Seconda Università degli Studi di Napoli, Napoli, Italy.
Endothelial cells labeled with Monoclonal Anti-Vimentin (Cat. No. V6630) (green) and Anti-JNK2 (red).
From Lara Milone, Biochimica e Biofisica, Seconda Università degli Studi di Napoli, Napoli, Italy.
Immunofluorescence
Endothelial cells labeled with Monoclonal Anti-Vimentin (Cat. No. V6630)(green) and Anti-p16 (red).
From Lara Milone, Biochimica e Biofisica, Seconda Università degli Studi di Napoli, Napoli, Italy.
Endothelial cells labeled with Monoclonal Anti-Vimentin (Cat. No. V6630)(green) and Anti-p16 (red).
From Lara Milone, Biochimica e Biofisica, Seconda Università degli Studi di Napoli, Napoli, Italy.
Immunohistochemistry
Staining of Stage XIII seminiferous tubule of an adult male rat following treatment with a testicular toxicant using Monoclonal Anti-Vimentin, clone No. V9 (Cat. No. V6630). The heavy staining surrounding the Sertoli cell nuclei indicates a collapse of the vimentin intermediate filament network following treatment. Interstitial cells are also stained.
From J. Wenz and M. Nakai, Univ. of Illinois, College of Med., Dept. of Veterinary Biosciences, Urbana, IL.
Staining of Stage XIII seminiferous tubule of an adult male rat following treatment with a testicular toxicant using Monoclonal Anti-Vimentin, clone No. V9 (Cat. No. V6630). The heavy staining surrounding the Sertoli cell nuclei indicates a collapse of the vimentin intermediate filament network following treatment. Interstitial cells are also stained.
From J. Wenz and M. Nakai, Univ. of Illinois, College of Med., Dept. of Veterinary Biosciences, Urbana, IL.
Immunofluorescence
Human osteosarcoma cells stained with Monoclonal Anti-Vimentin, clone No. V9 (Cat. No. V6630) using Biotinylated Anti-Mouse IgG and Avidin-Coumarin.
From M. Herzog and Dr. J. V. Small, Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
Human osteosarcoma cells stained with Monoclonal Anti-Vimentin, clone No. V9 (Cat. No. V6630) using Biotinylated Anti-Mouse IgG and Avidin-Coumarin.
From M. Herzog and Dr. J. V. Small, Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
Western Blotting Whole extract of HS-68 Human foreskin fibroblast cells were separated on SDS-PAGE and probed with Monoclonal Anti-Vimentin antibody produced in Mouse, Clone: V9 (Cat. No. V6630). Lanes 1. 1:200 antibody 2. 1:400 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | V9, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen ~58 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue |
| western blot: 1:200 using human fibroblasts HS-68 | |
| UniProt accession no. | P08670  |
| Application: | Monoclonal Anti-Vimentin antibody has been used in western blotting and immunocytochemistry. |
| Application: | Monoclonal Anti-Vimentin antibody produced in mouse has been used as a primary antibody to localize vimentin by immunohistochemistry (IHC) and for immunoprecipitation of vimentin. |
| Application: | Monoclonal Anti-Vimentin antibody produced in mouse has been used: • in immunofluorescence Analysis • in western blot • anti-vimentin antibody is injected into mature oocytes before embryo manipulations to bind endogenous vimentin (VIM) • in immunocytochemistry |
| Biochem/physiol Actions: | Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract. |
| Biochem/physiol Actions: | Vimentin helps to preserve the structure of cells and stability of tissue in mesenchymal cells. It is involved in tumorigenesis, EMT (epithelial mesenchymal transition) and the metastatic spread of cancer. It also controls infectious internalization of human papillomavirus 16 pseudovirions. |
| Biochem/physiol Actions: | Vimentin is the major cytoskeletal component in immature glia. It is an intermediate filament protein and serves as a substrate for several caspases. The cleavage of this protein by caspase dismantles intermediate filaments and promotes apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved a-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain. |
| Immunogen: | pig eye lens vimentin. |
| Other Notes: | This product can be found as purified product that was produced using cell culture hybridoma product. V6389 Anti-Vimentin antibody, Mouse monoclonal clone V9, purified from hybridoma cell culture |
| Specificity: | The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |