Vascular Endothelial Growth Factor human
SIGMA/V7259 - VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym: VEGF
CAS Number: 127464-60-2
MDL Number: MFCD00286542
Product Type: Chemical
SDS-PAGE Vascular Endothelial Growth Factor human (Cat. No. V7259) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of VEGF from rat plasma that elicits a 50% attachment factor in cell based Bioassay.
VEGF Pathway Research areas: Angiogenesis, Metastasis, Hypoxia, Osteogenesis, Developmental biology Disease areas: Cancer, Osteoporosis, Ischemic stroke, Inflammation, Rheumatoid Arthritis, Diabetes
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | powder |
| impurities | ≤0.1 EU/μg |
| mol wt | protein 38.2 kDa (as homodimer) |
| packaging | pkg of 10 μg |
| potency | 1-10 ng/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P15692  |
| Application: | Vascular Endothelial Growth Factor (VEGF) has been used for- • the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival • inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice |
| Biochem/physiol Actions: | Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen. VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation. |
| General description: | Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms. |
| Physical form: | Lyophilized from a sterile filtered buffered solution with no additives. |
| Physical properties: | Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |