Anti-VAMP-8 antibody produced in rabbit
SIGMA/V7514 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Endobrevin; Anti-
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-VAMP-8 antibody produced in Rabbit (Cat. No. V7514) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing VAMP-8 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:3000.
Immunoblotting Cell lysates were separated on SDS-PAGE and blotted with Anti-VAMP8 (Cat. No. V7514). The antibody was developed with Goat anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:3,000. Lanes 1,3. HEK293T cells transfected with human VAMP-8 2. HEK293T cells untransfected 3. Inhibition with VAMP-8 immunizing peptide (human, 38-56) (10 μg/mL)
Immunoprecipitation Rabbit Anti-VAMP8 (Cat. No. V7514) was used to immunoprecipitate VAMP8 from HeLa cells lysate. Detection antibody: Rabbit Anti-VAMP8 (N-terminal) (Cat. No. V7389), followed by Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg antibody-IP 2. Total cell lysate 3. Negative control: flowthrough 4. Negative control:1 μg antibody without lysate (The pictures are courtesy of Adi Abada Manelis and Zvulun Elazar, Faculty of Biology, Department of Biolmolecular Sciences, Weizmann Institute of Science, Israel)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~14 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 1-2 μg/mL using HEK-293T cells expressing human VAMP8. |
| UniProt accession no. | Q9BV40  |
| Application: | Anti-VAMP-8 antibody has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Vesicle-associated membrane protein 8 (VAMP-8/endobrevin) is as an endosomal v-SNARE (vesicles or granules-soluble NSF attachment protein receptors) involved in fusion between early and late endosomes. It can interact with syntaxin-7, syntaxin-8, and Vti1b (Vesicle transport through interaction with t-SNAREs homolog 1B) to form an endosomal fusion complex. VAMP8 interacts with syntaxin-2 and is involved in the terminal step of cytokinesis in mammalian cells. VAMP8 is the major vesicular v-SNARE of zymogen granules from pancreatic acinar cells and has been shown to be a general vesicular SNARE for regulated exocytosis of the exocrine system. For platelet release reaction VAMP8 is the primary v-SNARE. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vesicle-associated membrane protein 8 (VAMP-8/endobrevin) is located at 2p11.2 on the human chromosome. |
| Immunogen: | synthetic peptide corresponding to amino acids 38-56 of human VAMP8, conjugated to KLH. This sequence is highly conserved in mouse and rat VAMP8 (single amino acid substitution). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |