Monoclonal Anti-Vitronectin antibody produced in mouse
SIGMA/V7881 - clone VIT-2, ascites fluid
Synonym: Anti-V75
MDL Number: MFCD00162909
Product Type: Chemical
Western Blotting Purified Human plasma vitronectin were separated on SDS-PAGE and probed with Anti-Vitronectin antibody, Mouse monoclonal (Cat. No. V7881) followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:1,000 antibody 2. 1:2,500 antibody 3. 1:5,000 antibody 4. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | VIT-2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| mol wt | antigen 65-75 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable using human cultured fibroblasts | |
| microarray: suitable | |
| western blot: 1:2,500 using a denatured and reduced preparation of purified human plasma vitronectin | |
| UniProt accession no. | P04004  |
| Application: | Monoclonal Anti-Vitronectin antibody produced in mouse has been used in: • immunocytochemisry • western blotting • immunoprecipitation • immunofluorescence • enzyme-linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b-9 complex. Vitronectin protects thrombin from inactivation by antithrombin III in the presence of heparin, binds and stabilizes the activity of plasminogen activator inhibitor and mediates many other physiological functions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Vitronectin (mouse IgM isotype) is derived from the VIT-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human plasma vitronectin. Vitronectin is also referred as serum-spreading factor, S-protein of complement or epibolin. This monomeric acidic protein is one of the major multifunctional cell-adhesive glycoproteins in mammalian plasma and serum. This protein is usually seen as a mixture of 75 kDa and 65 kDa polypeptides. Human plasma and serum contain 0.1-0.4 mg/ml of vitronectin which is synthesized in the liver. It is also present in amniotic fluid and urine. |
| Immunogen: | human plasma vitronectin |
| Specificity: | By immunoblotting, the product shows no cross-reactivity with fibronectin, laminin, merosin, collagen type IV or chondroitin sulfate types A, B and C. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |