Monoclonal Anti-Vinculin antibody produced in mouse
SIGMA/V9131 - clone hVIN-1, ascites fluid
Synonym: Anti Vinculin; Vinculin Antibody; Vinculin Antibody - Monoclonal Anti-Vinculin antibody produced in mouse
MDL Number: MFCD00162905
Product Type: Chemical
Western Blotting JOURNAL CITATION: MicroRNA-145 targets YES and STAT1 in colon cancer cells. By: Gregersen, L. H., Jacobsen, A. B., et al. in PLoS One, 2010. PubMed ID: 20098684 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0008836), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Using confocal microscopy, human skin fibroblasts were labeled with Monoclonal Anti-Vinculin (Cat. No. V9131). The actin filaments were labeled with phalloidin (red) and hyaluronan was stained with fluorescently labeled hyaluronan binding protein (blue). Green color indicates focal contacts. From Kirsi Rilla, Biomedicine/Anatomy, University of Kuopio, Kuopio, Finland.
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat Heart sections stained with 15 μg/mL Anti-Vinculin antibody produced in Rabbit (Cat. No. V4139) (A). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 15 μg/mL Monoclonal Anti-Vinculin antibody produced in Mouse, Clone: hVIN1 (Cat. No. V9131) (B). The antibody was developed using Rabbit Anti-Mouse IgG-Cy3 conjugate antibody. Results Two Anti-Vinculin antibodies, V4139 (A) and V9131 (B), target different regions of Vinculin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Staining of a formaldehyde-fixed section of human myocardium using Monoclonal Anti-Human Vinculin, Clone No. hVIN-1 (Cat. No. V9131). Nuclei are stained with propidium iodide. Longitudinal section. All parts of the intercalated discs are stained. Lateral sarcolemma shows fine labeling as do the costameres close to the cell membrane.
From Prof. Dr. J. Schaper and Dr. S. Hein, Max Planck Institute, Bad Nauheim, Germany.
From Prof. Dr. J. Schaper and Dr. S. Hein, Max Planck Institute, Bad Nauheim, Germany.
Immunofluorescence
A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), Monoclonal Anti-Human Vinculin, clone No. hVIN-1 (Cat. No. V9131) (red) and Rabbit Anti-Pan Cadherin (Cat. No. C3678) (blue). Focal contacts appear yellow and intercellular junctions white.
From Prof. B. Geiger, Prof. Z. Kam and T. Volberg, Weizmann Institute of Science, Rehovot, Israel.
A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), Monoclonal Anti-Human Vinculin, clone No. hVIN-1 (Cat. No. V9131) (red) and Rabbit Anti-Pan Cadherin (Cat. No. C3678) (blue). Focal contacts appear yellow and intercellular junctions white.
From Prof. B. Geiger, Prof. Z. Kam and T. Volberg, Weizmann Institute of Science, Rehovot, Israel.
Immunofluorescence
Double labeling of cultured chicken fibroblasts for vinculin using Monoclonal Anti-Human Vinculin (Clone no. hVIN-1, Cat. No. V9131) and Rabbit Anti-Tubulin (Cat. No. T3526) (green).
From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg, Germany.
Double labeling of cultured chicken fibroblasts for vinculin using Monoclonal Anti-Human Vinculin (Clone no. hVIN-1, Cat. No. V9131) and Rabbit Anti-Tubulin (Cat. No. T3526) (green).
From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg, Germany.
Immunofluorescence
Staining of a formaldehyde-fixed section of human myocardium using Monoclonal Anti-Human Vinculin, Clone No. hVIN-1 (Cat. No. V9131). Nuclei are stained with propidium iodide. Transverse section. The sarcolemma of all cardiomyocytes are distinctly labeled. The intercalated discs appear as areas intensively stained within transversly sectioned myocytes. Yellow fluorescence is lipfuscin.
From Prof. Dr. J. Schaper and Dr. S. Hein, Max Planck Institute, Bad Nauheim, Germany.
Staining of a formaldehyde-fixed section of human myocardium using Monoclonal Anti-Human Vinculin, Clone No. hVIN-1 (Cat. No. V9131). Nuclei are stained with propidium iodide. Transverse section. The sarcolemma of all cardiomyocytes are distinctly labeled. The intercalated discs appear as areas intensively stained within transversly sectioned myocytes. Yellow fluorescence is lipfuscin.
From Prof. Dr. J. Schaper and Dr. S. Hein, Max Planck Institute, Bad Nauheim, Germany.
Immunofluorescence Primary human osteoblast cells labeled with Anti-Vinculin (Cat. No. V9131) (red), actin was stained with Phalloidin (Cat. No. P5282) (green) and nuclei stained blue. From Eng San Thian, Materials Science and Metallurgy, University of Cambridge, Cambridge, United Kingdom.
Immunofluorescence Primary human osteoblast cells labeled with Anti-Vinculin (Cat. No. V9131)(red). From Eng San Thian, Materials Science and Metallurgy, University of Cambridge, Cambridge, United Kingdom.
Immunofluorescence MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled with Monoclonal Anti-Vinculin (Cat. No. V9131) at a 1:400 dilution. From Inessa Skrypkina, Functional Genomics, Institute of Molecular Biology and Genetic of the National Academy of Sciences of Ukraine, Kiev, Ukraine.
Immunofluorescence
Human monocytes (J-111) labeled with Monoclonal Anti-Vinculin (Cat. No. V9131) (green). Actin filaments (red) and nuclear DNA (blue) were stained with Phalloidin-TRITC (Cat. No. P1951) and DAPI, respectively. Green-yellow color indicates focal contacts.
From Giuseppe Pani, Dipartmento di Scienze Fisiologiche, Biochimiche e Cellulari, Sassari University, Sassari, Italy.
Human monocytes (J-111) labeled with Monoclonal Anti-Vinculin (Cat. No. V9131) (green). Actin filaments (red) and nuclear DNA (blue) were stained with Phalloidin-TRITC (Cat. No. P1951) and DAPI, respectively. Green-yellow color indicates focal contacts.
From Giuseppe Pani, Dipartmento di Scienze Fisiologiche, Biochimiche e Cellulari, Sassari University, Sassari, Italy.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:200 Monoclonal Anti-Vinculin Clone: hVIN-1 (Cat. No. V9131). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. COS7 3. NIH-3T3 4. RAT2 5. CHO 6. MDBK 7. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | hVIN-1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| isotype | IgG1 |
| mol wt | antigen 116 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | frog, chicken, mouse, canine, human, bovine, rat, turkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): suitable |
| indirect immunofluorescence: 1:400 using cultured human fibroblasts | |
| western blot: 1:200 using extract of human fibroblasts | |
| UniProt accession no. | P18206  |
| Application: | Monoclonal Anti-Vinculin antibody produced in mouse has been used in: • western blotting • immunofluorescence staining • immunohistochemistry |
| Biochem/physiol Actions: | Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the β-subunit of integrin binds to talin. Talin binds to vinculin that interacts with α-actinin and possibly with itself. Since α-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions and the membrane-associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes. |
| Immunogen: | purified human vinculin from uterus. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Specificity: | Monoclonal Anti-Vinculin specifically stains vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells using indirect immunofluorescent labeling. The antibody reacts with the 116 kDa vinculin band in immunoblotting. The product reacts with vinculin of many species. Good reactivity is obtained with human, bovine, chicken, dog, rat, mouse, turkey, and Xenopus. The antibody shows cross reactivity with smooth muscle metavinculin. |
| Specificity: | Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |