Anti-Vinculin antibody, Mouse monoclonal
SIGMA/V9264 - clone hVIN-1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Vinculin antibody produced in mouse
MDL Number: MFCD00162905
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Arsenite-activated JNK signaling enhances CPEB4-Vinexin interaction to facilitate stress granule assembly and cell survival. By: Chang, Y. W. & Huang, Y. S. in PLoS One, 2014. PubMed ID: 25237887 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0107961), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells. By: Kudová, J., Prochazkova, J., et al. in PLoS One, 2016. PubMed ID: 27355368 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0158358), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Arsenite-activated JNK signaling enhances CPEB4-Vinexin interaction to facilitate stress granule assembly and cell survival. By: Chang, Y. W. & Huang, Y. S. in PLoS One, 2014. PubMed ID: 25237887 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0107961), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Monoclonal Anti-Vinculin Clone: hVIN-1 (Cat. No. V9264). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Vinculin Clone: hVIN-1 (Cat. No. V9264). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HS-68; 2. A431; 3. HeLa; 4. MDA-MB-231; 5. NIH-3T3; 6. Neuro-2a; 7. PC-12; 8. AT3B1
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | hVIN-1, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 116 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | frog, chicken, mouse, canine, human, bovine, rat, turkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: 0.05-0.1 μg/mL using HS-68 total cell extract | |
| UniProt accession no. | P18206  |
| Application: | Anti-Vinculin antibody, Mouse monoclonal has been used in • immunoblotting • immunofluorescence staining • immunocytochemistry • immunohistochemistry and enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle. |
| Immunogen: | purified human vinculin from uterus. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |