Anti-VPS34 antibody produced in rabbit
SIGMA/V9764 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Vps34 Antibody; Vps34 Antibody - Anti-VPS34 antibody produced in rabbit; Anti-PIK3C3; Anti-
Product Type: Chemical
Western Blotting JOURNAL CITATION: Disrupted autophagy after spinal cord injury is associated with ER stress and neuronal cell death. By: Liu, S., Sarkar, C., et al. in Cell Death Dis, 2015. PubMed ID: 25569099 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/cddis2014527), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Whole extract of human HEK-293T cells overexpressing human VPS34 was separated on SDS-PAGE and probed with Rabbit Anti-VPS34 (Cat. No. V9764). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + VPS34 immunizing peptide (human, 22-34)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~105 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 2-4 μg/mL using whole extracts of HEK-293T cells expressing human VPS34 | |
| UniProt accession no. | Q8NEB9  |
| Application: | Anti-VPS34 antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | Vacuolar protein sorting 34 (VPS34) is a lipid kinase that phosphorylate phosphatidylinositol (PtdIns), to form phosphatidylinositol 3 phosphate (PtdIns3P). VPS34 plays a vital role in various intracellular trafficking events such as autophagy and phagosome formation. Thus, the inhibition of kinase activity of Vps34 might be considered as a potential therapeutic method for cancer treatment. VPS34 is also involved in internal vesicle formation within multivesicular endosomes, reverse transport of endosome to Golgi apparatus and membrane transport from the outer nuclear membrane towards the cell periphery. Mutation in the gene encoding VPS34 is associated with pathogenesis of schizophrenia. VPS34 plays an essential role in nutrient/amino acid induced activation of mechanistic target of rapamycin (mTOR) signaling in cultured cells and it also facilitates early embryogenesis and cell proliferation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vacuolar protein sorting 34 (VPS34), also known as phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), is encoded by the gene mapped to human chromosome 18q12.3. The encoded protein belongs to the phosphoinositide (PI)-3-kinase family. VPS34 is widely expressed in all tissues of mammals. |
| Immunogen: | synthetic peptide corresponding to amino acids 22-34 of human VPS34, conjugated to KLH via an added cysteine residue. The corresponding sequence is identical in mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Target description: | VPS34 is known to be involved in endocytosis and vesiculartrafficking. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |