Viral RNA Extraction Buffer
SIGMA/VRE100 - One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva
Synonym: For use with Reverse Transcription-qPCR; Viral Lysis Solution for RT-qPCR; Viral RNA Extraction
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| concentration | 4X |
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | LAMP: suitable |
| PCR: suitable | |
| qPCR: suitable | |
| RT-PCR: suitable |
| Application: | The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, and HEX. RT-qPCR compatibility has been verified with several one-step RT-qPCR kits: • Quantitative RT-PCR ReadyMix™ (Sigma-Aldrich, catalog QR0200) • KAPA PROBE FAST One-Step Universal (Sigma-Aldrich, catalog KK4752) • KiCqStart® One-Step Probe RT-qPCR ReadyMix™ (Sigma-Aldrich, catalog KCQS07) • TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525) The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays. |
| Features and Benefits: | • Extracted viral RNA can be used for detection without cleanup • Directly compatible with quantitative reverse transcription polymerase chain reaction (qRT-PCR) and loop mediated isothermal amplification(LAMP) assays • Ideal for enveloped RNA viruses in saliva, saline, Amies medium, and viral transport medium • Highly sensitive and faster than other leading methods, lysis by Proteinase K and heating |
| General description: | The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity. |
| Legal Information: | KiCqStart is a registered trademark of Qiagen Beverly Inc. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |