Western Blocker™ Solution
SIGMA/W0138 - for HRP detection systems
Synonym: Western blot blocking solution; blocking buffer
Product Type: Chemical
| Quality Level | 200  |
| shelf life | ≥2 yr |
| storage temp. | 2-8°C |
| Application: | Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems. Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot. |
| Features and Benefits: | • Does not inhibit weak HRP signals, as many milk-based blockers do. • Can be used both as a blocking component and as a probing component. • Supplied ready-to-use; no preparative step is required. |
| General description: | Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies. |
| Legal Information: | Western Blocker is a trademark of Sigma-Aldrich Co. LLC |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |