Anti-WAVE antibody produced in rabbit
SIGMA/W0392 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-WASF1; Anti-Wiskott-Aldrich syndrome protein family member 1
MDL Number: MFCD04118689
Product Type: Chemical
Immunoblotting
Tissue lysates were separated on SDS-PAGE and blotted with Anti-WAVE (Cat. No. W0392). The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Antibody dilution 1:4,000
Lanes
1: Mouse brain (cytosolic fraction S1)
2: Rat brain (cytosolic fraction S1)
3: Rat brain (cytosolic fraction S1). Inhibition of antibody binding with WAVE-1 immunizing peptide (human, 540-559) (20 μg/mL)
Tissue lysates were separated on SDS-PAGE and blotted with Anti-WAVE (Cat. No. W0392). The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Antibody dilution 1:4,000
Lanes
1: Mouse brain (cytosolic fraction S1)
2: Rat brain (cytosolic fraction S1)
3: Rat brain (cytosolic fraction S1). Inhibition of antibody binding with WAVE-1 immunizing peptide (human, 540-559) (20 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 75 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | microarray: suitable |
| western blot: 0.2-0.4 μg/mL using rat and mouse brain cytosolic fraction (S1) | |
| UniProt accession no. | Q92558  |
| Application: | Anti-WAVE antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | WAVE proteins also play key roles in the induction of various actin remodelling processes including membrane ruffling and lamellipodia formation. WAVE proteins bind to phosphatidylinositol-3,4, |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The WASP family members include WASP, N-WASP and WAVE (WASP-family verprolin homologous protein) proteins. WAVE proteins (also termed SCAR, WASF proteins), include three isoforms WAVE-1, WAVE-2 and WAVE-3. Expression of WAVE-1 and WAVE-3 isoforms is restricted to the brain, while WAVE-2 is ubiquitously expressed. WAVE-1-3 have structures like WASP and N-WASP; these include two conserved domains: a WAVE homology domain (WHD), also known as SCAR homology domain (SHD), a C-terminal acidic domain that binds to the Arp2/3 complex; and a central core domain composed of proline-rich sequences that interact with cofilin and various SH3 proteins. |
| Immunogen: | synthetic peptide corresponding to amino acids 540-559 located at the C-terminus of human WAVE-1. The sequence is identical in several species, including mouse and rat WAVE-1 and is conserved (75% sequence identity) in other WAVE isoforms. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |